Cryo-EM Studies of Virus-Antibody Immune Complexes

作     者：Na Li Zhiqiang Li Yan Fu Sheng Cao Na Li;Zhiqiang Li;Yan Fu;Sheng Cao

作者机构：CAS Key Laboratory of Special Pathogens and BiosafetyWuhan Institute of VirologyWuhan 430071China Center for Biosafety Mega-ScienceChinese Academy of SciencesWuhan 430071China University of Chinese Academy of SciencesBeijing 100049China 

出 版 物：《Virologica Sinica》 (中国病毒学（英文版）)

年 卷 期：2020年第35卷第1期

页      面：1-13页

核心收录：

学科分类：1001[医学-基础医学(可授医学、理学学位)] 100102[医学-免疫学] 10[医学] 

基　　金：the National Natural Science Foundation of China(Grant Nos.31570161 and 31770169) the“One-Three-Five”Strategic Programs of the Wuhan Institute of Virology,Chinese Academy of Sciences(Grant No.Y605211SA3) 

主　　题：Cryo-electron microscopy(Cryo-EM) Icosahedral Antigen Virion Immune complex 

摘      要：Antibodies play critical roles in neutralizing viral infections and are increasingly used as therapeutic drugs and diagnostic tools. Structural studies on virus-antibody immune complexes are important for better understanding the molecular mechanisms of antibody-mediated neutralization and also provide valuable information for structure-based vaccine ***-electron microscopy(cryo-EM) has recently matured as a powerful structural technique for studying bio-macromolecular complexes. When combined with X-ray crystallography, cryo-EM provides a routine approach for structurally characterizing the immune complexes formed between icosahedral viruses and their antibodies. In this review, recent advances in the structural understanding of virus-antibody interactions are outlined for whole virions with icosahedral T = pseudo 3(picornaviruses) and T = 3(flaviviruses) architectures, focusing on the dynamic nature of viral shells in different functional states. Glycoprotein complexes from pleomorphic enveloped viruses are also discussed as immune complex antigens. Improving our understanding of viral epitope structures using virus-based platforms would provide a fundamental road map for future vaccine development.