Dipyridamole enhances inhibitory effect of adenosine on neutrophils in human peripheral blood

作     者：Yu Zhang Qingyan Zhang Jing Zhang Qian Tian minghua Li 

作者机构：Department of Cardiology the First Hospital of Jilin University Changchunl 30021 Jilin Province China 

出 版 物：《Journal of Nanjing Medical University》 (南京医科大学学报（英文版）)

年 卷 期：2008年第22卷第4期

页      面：243-245页

学科分类：1001[医学-基础医学(可授医学、理学学位)] 10[医学] 

主　　题：dipyridamole hydrogen peroxide adenosine neutrophils 

摘      要：Objective： It was known that neutrophils play an important role in ischemic-reperfusion injury. In this study we tested the effect and its mechanism of dipyfidamole on neutrophils. Methods：Hydrogen peroxide（H2O2） production by neutrophils was determined using luminol amplified chemiluminescence and the percentage of activity was calculated by observing the uninhibited peak height. Results： Dipyfidamole per se produced a concentration-dependent inhibition of H2O2 by formyl-MetleuPhe（fMLP）-stimulated neutrophils. Dipyridamole at a low concentration（0.3 μ mol · L^-1） that per se affected neutrophils only slightly, enhanced markedly the effects of adenosine on neutrophils. On the other hand, dipyfidamole did not alter the inhibitory effect of NECA（5＇ -N-ethylcarboxamidoadenosine） on neutrophils. However, propentofylline, a known inhibitor of adenosine uptake, also gotten the same result. Conclusion：Dipyfidamole inhibited the production of H2O2 by fMLP-sfimulated neutrophils. Dipyfidamole at a low concentration enhanced the inhibitory effect of adenosine on neutrophils. The mechanism involved is probably due to the effect of dipyfidamole on adenosine uptake.