Bone morphogenetic protein 2 improves patellar tendon healing by promoting migration and proliferation of tenocytes

作     者：TANG MeiKuen LEE Kenneth K 

作者机构：Key Laboratory for Regenerative Medicine of Ministry of Education Jinan University Joint Laboratory for Regenerative Medicine the Chinese University of Hong Kong-Jinan University School of Biomedical Sciencesthe Chinese University of Hong Kong International Base of Collaboration for Science and Technology (JNU) the Ministry of Science and Technology & Guangdong Province School of Biomedical Sciences the Chinese University of Hong Kong 

出 版 物：《Chinese Science Bulletin》 (Chinese Science Bulletin)

年 卷 期：2011年第56卷第13期

页      面：1361-1369页

核心收录：

学科分类：1002[医学-临床医学] 100210[医学-外科学(含：普外、骨外、泌尿外、胸心外、神外、整形、烧伤、野战外)] 10[医学] 

基　　金：supported by the National Natural Science Foundation of China (30770886,30570369,30340038,30973158) National High Technology Research and Development Program of China (2007AA02Z105) Natural Science Foundation of Guangdong Province (04105826) Key Science and Technology Program of Guangdong Province (2004B3-0601007) Key Science and Technology Program of Guangzhou (2006Z3-E5251) the Fundamental Research Funds for the Central Universities(21609408) 

主　　题：骨形态发生蛋白 肌腱细胞 愈合能力 迁移 扩散 BMP 表达模式 原位杂交 

摘      要：The repair of injured tendons remains a great challenge because of the poor intrinsic healing capacity of tendons. In this study, we examined the spatiotemporal expression pattern of the bone morphogenetic protein 2 (bmp-2) gene in normal and experimentally injured rat patellar tendons. We also investigated the ability of exogenously applied BMP-2 to promote patellar tendon healing. In situ hybridization with bmp-2 and alk-6 (bmp-2 receptor) antisense riboprobes revealed that both genes were normally expressed at low levels in intact rat tendons. However, bmp-2 expression was significantly upregulated in tenocytes found in the wound site at 7 d and later following tendon injury. In addition, it was found that bmp-2 was expressed in cultured patellar tenocytes. Appli- cation of exogenous BMP-2 to the tendon wound site significantly enhanced tendon repair. Moreover, in vitro and in vivo studies further demonstrated that BMP-2 enhanced tenocyte proliferation and migration to the wound site. The detectable amount of BMP-2 in normal tendons suggests that a basal level of bmp-2 expression was likely present to maintain the normal functions of the patellar tendon. Injury to the tendon induced increased bmp-2 expression intrinsically, but the expression level was insufficient for proper tendon repair. Our findings indicate that it is possible to significantly improve tendon healing by applying exogenous BMP-2 to the wound site, inferring that this protein could be developed as a potential therapeutic reagent for the treatment of damaged tendons.