Mesenchymal stem cell enhances chondral defects healing in horses

作     者：Ana Lucia Miluzzi Yamada Armando de Mattos Carvalho Andrei Moroz Elenice Deffune Marcos Jun Watanabe Carlos Alberto Hussni Celso Antonio Rodrigues Ana Liz Garcia Alves 

作者机构：Department of Veterinary Surgery and AnesthesiologySchool of Veterinary Medicine and Animal Science(FMVZ)UNESPUniversidade Estadual PaulistaBotucatuBrazil Faculty of Veterinary MedicineCuiaba UniversityCuiabaBrazil Department of MorphologyInstitute of BiosciencesUNESPBotucatuBrazil Blood CenterSchool of MedicineUNESPBotucatuBrazil 

出 版 物：《Stem Cell Discovery》 (干细胞探索（英文）)

年 卷 期：2013年第3卷第4期

页      面：218-225页

学科分类：1002[医学-临床医学] 100214[医学-肿瘤学] 10[医学] 

基　　金：financially supported by the FAPESP(Sao Paulo Research Foundation)(2008/56360-7 and 2009/06059-1). 

主　　题：Arthroscopy Cartilage Equine Lameness Mesenchymal Stem Cells 

摘      要：The purpose of this study was to evaluate the effect of intralesional Mesenchymal Stem Cells (MSC) on the treatment of experimentally induced articular chondral defects in horses, emphasizing the benefits of this application in veterinary medicine. Chondral defects were induced in the medial femoral trochlea of both hind limbs of four horses. Thirty days post induction;the horses were divided into two groups. The G1 was submitted to treatment with MSC and the G2 was the control group. Clinical evaluations, synovial fluid analysis and synovial Prostaglandin E2 (PGE2) assessment were performed prior to defects and fortnightly up to 120 and 150 days. Macroscopic, histopathological and histochemical evaluations were performed at the end of the experiment. The treatment with MSC reduced the intraarticular inflammatory process. The G1 showed lower PGE2 concentrations in the synovial fluid and greater percentage of mononuclear cells and lower percentages of lymphocytes and neutrophils. The treatment improved the macro and microscopic aspects of repair tissue. No difference was observed in the scores of lameness between the G1 and G2. The use of MSC in the treatment of chondral defects minimized joint inflammation, as confirmed by synovial fluid analysis. The treatment resulted in an improved repair tissue, verified by macroscopic examination, histochemical and histopathological analysis.