Glutathione Production by <i>Yarrowia lipolytica</i>Showing Both Methyglyoxal Resistance and a High Activity of Glutathione Synthetase

作     者：Naofumi Shiomi Emi Harada Risako Nabeshima 

作者机构：Graduate School of Human Sciences Kobe College Kobe Japan 

出 版 物：《Advances in Microbiology》 (微生物学（英文）)

年 卷 期：2012年第2卷第2期

页      面：171-180页

学科分类：1002[医学-临床医学] 100214[医学-肿瘤学] 10[医学] 

主　　题：Glutathione Yarrowia lipolytica Glutathione Synthetase GSH2 Methylglyoxal 

摘      要：Although Yarrowia lipolytica is an important host strain, there have so far been few studies on the production of glutathione by the strain. We therefore performed a study to obtain an improved strain of Y. lipolytica ATCC20688, which could produce a high yield of glutathione. First, the capability of glutathione production in the ATCC20688 strain was estimated. In comparison with other yeasts, the yield of this strain was higher than those in Pichia strains. Furthermore, this strain could produce glutathione by assimilating sodium oleate. We next performed mutation and gene cloning to improve the yield. After the yield of glutathione was improved in the isolated methylglyoxal-resistant mutant (MGR3), the glutathione synthetase gene was cloned into the MGR3 strain. By using this recombinant strain, we could reach the maximum yield and intracellular content of glutathione of 54 mg/L-medium and 30 mg/g-dry cell weight, respectively.