Fermentation of the Straw Material Paja Brava by the Yeast <i>Pichia stipitis</i>in a Simultaneous Saccharification and Fermentation Process
Fermentation of the Straw Material Paja Brava by the Yeast <i>Pichia stipitis</i>in a Simultaneous Saccharification and Fermentation Process作者机构：Department of Chemical Engineering Federal University of Pernambuco Recife Brazil Department of Chemical Engineering Lund University Lund Sweden
出 版 物：《Journal of Sustainable Bioenergy Systems》 (可持续生物质能源系统（英文）)
年 卷 期：2013年第3卷第2期
页 面：99-106页
学科分类：1002[医学-临床医学] 100214[医学-肿瘤学] 10[医学]
主 题：Ethanol Lignocellulose Fermentation Xylose
摘 要：Paja Brava is a native South American grass with a high carbohydrate content. In the current work, the potential of using this feedstock for ethanol production using a simultaneous saccharification and fermentation (SSF) process with the xylose-fermenting yeast Pichia stipitis (Scheffersomyces stipitis) CBS6054 was investigated. The straw material was subjected to SO2 catalyzed steam pretreatment at 200°C and 5 min residence time, which resulted in a solubilization of pentose sugars (mainly xylose) of 64% with only minor amounts of degradation products. The obtained material, including the pretreatment liquid, was subsequently hydrolyzed and fermented in an SSF process at microaerobic conditions using either a batch or a fed-batch process at a total water-insoluble solids loading of 10%. Overall yields of ethanol based on all available sugars of 0.24 g/g and 0.27 g/g were obtained for batch and fed-batch mode of operation, respectively. The higher yield in the fed-batch process coincided with a higher degree of conversion of the sugars in the liquid medium, in particular of arabinose, for which the conversion was doubled (from 48% to 97%).
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