Fixation of hybrid vigor in rice:synthetic apomixis generated by genome editing
作者机构：State Key Laboratory of Rice BiologyChina National Rice Research InstituteChinese Academy of Agricultural SciencesHangzhou 310006China
出 版 物：《aBIOTECH》 (生物技术通报（英文版）)
年 卷 期：2020年第1卷第1期
页 面：15-20页
核心收录：
主 题：Apomixis Heterosis Hybrid vigor Genome editing
摘 要：Apomixis is an asexual reproduction process in which clonal seeds are formed without meiosis and *** of its potential in permanently preserving hybrid vigor,apomixis has attracted a great deal of interests from plant biologists and the seed ***,despite of decades of effort,introgression of apomixis traits from wild relatives into major crops has remained ***,synthetic apomixis has been proposed as an alternative to fix hybrid *** this article,I present the development of the MiMe(Mitosis instead of Meiosis),which turns meiosis into mitosis and leads to the production of clonal ***-like clonal seeds are generated when MiMe plants are crossed to special genome elimination lines,which contain an altered centromere-specific histone 3(CENH3).Furthermore,induction of haploid plants from egg cells can be achieved by either egg cell-specific expression of BABY BOOM1(BBM1),or disruption of MATRILINEAL(MTL)using CRISPR/Cas9 gene-editing *** apomixis is established and clonal seeds are produced by simultaneous engineering MiMe with altering BBM1 expression or MTL ***,I discuss how to further improve the apomixis strategy and its applications in crop breeding.
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