Precise gene replacement in plants through CRISPR/Cas genome editing technology:current status and future perspectives

作     者：Shaoya Li Lanqin Xia Shaoya Li;Lanqin Xia

作者机构：Institute of Crop Sciences(ICS)Chinese Academy of Agricultural Sciences(CAAS)Beijing 100081China 

出 版 物：《aBIOTECH》 (生物技术通报（英文版）)

年 卷 期：2020年第1卷第1期

页      面：58-73页

核心收录：

学科分类：0710[理学-生物学] 07[理学] 09[农学] 071007[理学-遗传学] 0901[农学-作物学] 0836[工学-生物工程] 090102[农学-作物遗传育种] 

基　　金：Some mentioned works in this review are partly funded by the Ministry of Agriculture of China(Grant nos.2019ZX08010001 and 2019ZX08010003) the Central Non-Profit Fundamental Research Funding supported by Institute of Crop Sciences,Chinese Academy of Agricultural Sciences(S2018QY05) 

主　　题：CRISPR/Cas Gene targeting(GT) Gene/allele replacement Genome editing Homology-directed repair(HDR) 

摘      要：CRISPR/Cas,as a simple,versatile,robust and cost-effective system for genome manipulation,has dominated the genome editing field over the past few *** application of CRISPR/Cas in crop improvement is particularly important in the context of global climate change,as well as diverse agricultural,environmental and ecological *** CRISPR/Cas toolboxes have been developed and allow for targeted mutagenesis at specific genome loci,transcriptome regulation and epigenome editing,base editing,and precise targeted gene/allele replacement or tagging in *** particular,precise replacement of an existing allele with an elite allele in a commercial variety through homology-directed repair(HDR)is a holy grail in genome editing for crop improvement as it has been very difficult,laborious and time-consuming to introgress the elite alleles into commercial varieties without any linkage drag from parental lines within a few generations in crop breeding ***,it still remains very challenging in crop *** review intends to provide an informative summary of the latest development and breakthroughs in gene replacement using CRISPR/Cas technology,with a focus on achievements,potential mechanisms and future perspectives in plant biological science as well as crop improvement.