Mechanism of acupuncture on anti-neuronal apoptosis and the regulation of PI3K/AKT signaling pathway in heroin relapse rats

作     者：Xiao-Rong Hou Xing-Hui Cai Sheng-Bing Wu Rong-Jun Zhang Li-Da Zhang Xiao-Ge Song 侯晓蓉;蔡兴慧;吴生兵;张荣军;张利达;宋小鸽

作者机构：College of Integrated Chinese and Western MedicineAnhui University of Traditional Chinese MedicineHefei 230012China Institute of Acupuncture-Moxibustion and MeridianAnhui University of Traditional Chinese MedicineHefei 230038China Scientific Research Experiment CenterAnhui University of Traditional Chinese MedicineHefei 230038China College of GraduateAnhui University of Traditional Chinese MedicineHefei 230038China 

出 版 物：《World Journal of Acupuncture-Moxibustion》 (世界针灸杂志（英文版）)

年 卷 期：2019年第29卷第4期

页      面：285-289页

核心收录：

学科分类：1005[医学-中医学] 100512[医学-针灸推拿学] 10[医学] 

基　　金：Supported by Youth Fund Project of National Natural Science Foundation of China: 81503658 Key Project of Natural Science Research in Universities of Anhui Province: KJ2017A289 Anhui University Scientific Research Platform Construction Project: 2015TD033 Exploration Project of Anhui University of Traditional Chinese Medicine: 2016ts078 

主　　题：Acupuncture Heroin relapse rats Neuronal apoptosis PI3K/AKT signaling pathway 

摘      要：Objective:To explore the mechanism of acupuncture intervention on anti-neuronal apoptosis in heroindependent rats based on the PI3K/AKT signaling ***:A total of 30 SD rats were randomly divided into a normal group,a model group and an acupuncture group,10 rats in each *** the model group and the acupuncture group,the heroin relapse rat model was established by intramuscular incremental injection of *** the model group,no any intervention was *** the acupuncture group,after modeling,acupuncture was applied atBǎihuì(百会GV20)andDàzhuī(大椎GV14).Transmission electron microscope(TEM)was adopted to observe neuronal apoptosis in the *** effect of acupuncture on anti-neuronal apoptosis was *** reverse transcription-polymerase chain reaction(RT-PCR)and western blot methods,the mRNA and protein expressions of PI3 K and AKT were detected in ventral tegmental area(VTA).Results:It was found that edema was presented in VTA neuronal cytoplasm and organelles basically disappeared in the heroin relapse rats,nuclear chromatin aggregation,condensation and increased neuronal apoptosis were presented as *** acupuncture atBǎihuì(百会GV20)andDàzhuī(大椎GV14),a small amount of mitochondria,rough endoiplasmic reticulum and glycogen granules were visible in the cytoplasm of VTA *** nuclear membrane structure was clear and the chromatin in the nucleus was basically *** fluorescence quantitative polymerase chain reaction(PCR)and western blot methods were adopted to detect mRNA and protein expressions of PI3K and *** was found that the mRNA and protein expressions of PI3K and AKT in the brain of the rats in the model group were lower than those in the normal group(all P0.05).Compared with the model group,the mRNA and protein expressions of PI3K and AKT in the acupuncture group were increased(all P0.05),tending to the levels as the normal ***:The effect of acupuncture on anti-brain cell apoptosis in heroin relap