Changes in neurological and pathological outcomes in a modified rat spinal cord injury model with closed canal

作     者：Xin Sun Xing-Zhen Liu Jia Wang Hai-Rong Tao Tong Zhu Wen-Jie Jin Kang-Ping Shen Xin Sun;Xing-Zhen Liu;Jia Wang;Hai-Rong Tao;Tong Zhu;Wen-Jie Jin;Kang-Ping Shen

作者机构：Shanghai Key Laboratory of Orthopedic ImplantsDepartment of Orthopedic SurgeryShanghai Ninth People’s HospitalShanghai Jiao Tong University School of MedicineShanghaiChina Department of PathologyShanghai Xinhua HospitalShanghai Jiao Tong University School of MedicineShanghaiChina 

出 版 物：《Neural Regeneration Research》 (中国神经再生研究（英文版）)

年 卷 期：2020年第15卷第4期

页      面：697-704页

核心收录：

学科分类：0710[理学-生物学] 100208[医学-临床检验诊断学] 1002[医学-临床医学] 1001[医学-基础医学(可授医学、理学学位)] 100104[医学-病理学与病理生理学] 10[医学] 

主　　题：Basso-Beattie-Bresnahan scores closed spinal canal HIF-1α hypoxia model nerve regeneration open spinal canal rat secondary injury spinal cord injury VEGF 

摘      要：Most animal spinal cord injury models involve a laminectomy,such as the weight drop model or the transection ***,in clinical practice,many patients undergo spinal cord injury while maintaining a relatively complete spinal ***,open spinal cord injury models often do not simulate real injuries,and few previous studies have investigated whether having a closed spinal canal after a primary spinal cord injury may influence secondary ***,we aimed to assess the differences in neurological dysfunction and pathological changes between rat spinal cord injury models with closed and open spinal ***-Dawley rats were randomly divided into three *** the sham group,the tunnel was expanded only,without inserting a screw into the spinal *** the spinal cord injury with open canal group,a screw was inserted into the spinal canal to cause spinal cord injury for 5 minutes,and then the screw was pulled out,leaving a hole in the vertebral *** the spinal cord injury with closed canal group,after inserting a screw into the spinal canal for 5 minutes,the screw was pulled out by approximately 1.5 mm and the flat end of the screw remained in the hole in the vertebral plate so that the spinal canal remained closed;this group was the modified model,which used a screw both to compress the spinal cord and to seal the spinal *** 7 days post-operation,the Basso-Beattie-Bresnahan scale was used to measure changes in neurological ***-eosin staining was used to assess *** evaluate the degree of local secondary hypoxia,immunohistochemical staining and western blot assays were applied to detect the expression of hypoxia-inducible factor 1α(HIF-1α)and vascular endothelial growth factor(VEGF).Compared with the spinal cord injury with open canal group,in the closed canal group the Basso-Beattie-Bresnahan scores were lower,cell morphology was more irregular,the percentage of morphologically normal neurons was lower