Genotype identification of Orientia tsutsugamushi isolated from Nan Peng Lie Islands in China

作     者：彭桂福 王志斌 王珊珊 黄佳亮 姜普林 曾年华 刘金华 朱少凡 PENG Guifu;Wang Zhibing;WANG Shanshan;Huang Jialiang;JIANG Pulin;Zeng Nianhua;Liu Jinhua;ZHU Shaofan

作者机构：广州军区军事医学研究所流行病研究室广州510507 

出 版 物：《Chinese Medical Journal》 (中华医学杂志（英文版）)

年 卷 期：2002年第115卷第12期

页      面：1881-1882,155页

核心收录：

学科分类：1004[医学-公共卫生与预防医学(可授医学、理学学位)] 1002[医学-临床医学] 100401[医学-流行病与卫生统计学] 10[医学] 

主　　题：Animals China DNA, Bacterial Genotype Mice Orientia tsutsugamushi Polymorphism, Restriction Fragment Length Rats 

摘      要：OBJECTIVE: To identify genotype of eight strains of Orientia tsutsugamushi (O. tsutsugamushi) isolated from Nan Peng Lie Islands in China and establish tsutsugamushi disease nature foci for this region. METHODS: The nested polymerase chain reaction and restriction fragment length polymorphism (PCR-RFLP) were used. Three primers were selected from the DNA sequence of the gene encoding type-specific 56-kDa protein of the Karp strain. The positive products were digested by Hine II and Pst I, meanwhile profiles specific to each strain were analyzed. RESULTS: Three genotypes of O. tsutsugamushi including Karp, Kato and a new strain existed on Nan Peng Lie Islands. CONCLUSION: Nan Peng Lie Islands is tsutsugamushi disease nature foci.