Moxibustion therapy improving delayed memory deficits via promoting neurogenesis and angiogenesis of hippocampus in a vascular dementia rat model

作     者：Fan Yin-qiu Yang Jun Cui Jing-cheng Wang Pin Li Yue Gui Li 樊吟秋;杨骏;崔竟成;王频;李悦;桂利

作者机构：Graduate SchoolAnhui University of Chinese MedicineHefei 230031China The First Affiliated HospitalAnhui University of Chinese MedicineHefei 230031China Clinical Department of Acupuncture-moxibustion and OrthopaedicAnhui University of Chinese MedicineHefei 230031China 

出 版 物：《Journal of Acupuncture and Tuina Science》 (针灸推拿医学(英文版))

年 卷 期：2019年第17卷第6期

页      面：383-394页

核心收录：

学科分类：1006[医学-中西医结合] 1005[医学-中医学] 10[医学] 

基　　金：国家自然科学基金项目 No.81473785 No.30973801 

主　　题：Moxibustion Therapy Point Baihui(GV 20) Point Shenting(GV 24) Point Dazhui(GV 14) Governor Vessel Dementia Vascular Rats 

摘      要：Objective:To investigate the alteration of delayed memory and its relationship with neurogenesis and angiogenesis in vascular dementia(VD)rats after moxibustion ***:Two hundred adult male SPF Wistar rats were chosen for the ***-six rats were randomly selected as the sham operation *** for rats in the sham operation group(n=36),the others were made into VD models by bilateral comm on carotid arteries occlusi on(BCCAo).After modeli ng,the 108 survived rats were ran domly divided into 3 groups:a model group,a neural stem cells(NSCs)plus endothelial progenitor cells(EPCs)moxibustion group and a NSCs moxibustion ***-transplanted implant was transplanted into the rats in the NSCs plus EPCs moxibustion group,and the rats in the NSCs moxibustion group were transplanted by NSCs *** NSCs plus EPCs moxibustion group and the NSCs moxibustion group received suspended moxibustion therapy at Baihui(GV 20),Dazhui(GV 14)and Shenting(GV 24),(each group was divided into 3 subgroups by the treatment course as 1,2 and 3 courses).Every group was measured by Morris water maze to evaluate its delayed memory after 3 treatment courses and the rat s brain was taken out after perfusion of 4%paraformaldehyde one day after 1,2 and 3 treatment courses,*** protein expression was detected by laser con focal microscope to an a lyze the effect on neuroge nesis and an gioge ***:VD rats showed delayed memory in Morris water maze test 3 d after ischemic *** 3 courses of moxibustion therapy,VD-induced delayed memory deficits were improved in the NSCs plus EPCs moxibustion group and the NSCs moxibustion *** expressions of nestin,doublecortin(DCX)and CD34 increased significantly in the two moxibusiton groups after every treatment course(all P0.05),which might contribute to the neurogenesis and angiogenesis in *** addition,compared with the rats in the NSCs moxibustion group,the expressions of nestin,DCX and CD