DNA methylome study of human cerebellar tissues identified genes and pathways possibly involved in essential tremor
作者机构：Center for GenomicsSchool of MedicineLoma Linda UniversityLoma LindaCA 92350USA Division of Movement DisordersDepartment of NeurologyLoma Linda University Medical CenterLoma LindaCA 92350USA Department of Basic SciencesSchool of MedicineLoma Linda UniversityLoma LindaCA 92350USA
出 版 物：《Precision Clinical Medicine》 (精准临床医学（英文）)
年 卷 期：2019年第2卷第4期
页 面：221-234页
学科分类：1002[医学-临床医学] 100214[医学-肿瘤学] 10[医学]
基 金：Our RRBS fastq files were submitted to GEO(Access number:GSE134426)
主 题：DNA methylome reduced representation bisulfite sequencing essential tremor cerebellum epigenetics CpGs
摘 要：Background:Essential tremor(ET)is a neurological syndrome of unknown origin with poorly understood etiology and *** is suggested that the cerebellum and its tracts may be involved in the pathophysiology of *** methylome interrogation of cerebellar tissue may help shine some light on the understanding of the mechanism of the development of *** study used postmortem human cerebellum tissue samples collected from 12 ET patients and 11 matched non-ET controls for DNA methylome study to identify differentially methylated genes in ***:Using Nugen’s Ovation reduced representation bisulfite sequencing(RRBS),we identified 753 genes encompassing 938 CpG sites with significant differences in DNA methylation between the ET and the control *** genes were further analyzed with Ingenuity Pathway Analysis(IPA)by which we identified certain significant pathways,upstream regulators,diseases and functions,and networks associated with ***:Our study provides evidence that there are significant differences in DNA methylation patterns between the ET and control samples,suggesting that the methylation alteration of certain genes in the cerebellum may be associated with ET *** identified genes allude to the GABAergic hypothesis which supports the notation that ET is a neurodegenerative disease,particularly involving the cerebellum.
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