A Rapid and Efficient Method for Isolating High Quality Total RNA from Edible Fungi

作     者：SHUI Pirong,ZHENG Xiaobing,LIN Junfang,GUO Liqiong Department of Bioengineening,College of Food Sciences,South China Agricultural University,Guangzhou,Guangdong 510640,China 

出 版 物：《食用菌学报》 (Acta Edulis Fungi)

年 卷 期：2008年第1期

页      面：37-41页

学科分类：09[农学] 0902[农学-园艺学] 090202[农学-蔬菜学] 

基　　金：Sponsored by the National Natural Science Foundation ( No 30671457and30371000) the Natural Science Foundation of Guangdong Province (No 5006680and032239) 

主　　题：edible fungi total RNA isolation STE method 

摘      要：A rapid and efficient procedure,the STE(sodium chloride-Tris-HCl-EDTA) method,for isolating high quality total RNA from polysaccharide-rich mycelia of the edible fungi Lentinus edodes,Ganoderma lucidum and a Polyporus *** *** quality of the total RNA isolated by the STE method was superior to that obtained using the Trizol *** A260/A280 and A260/A230 ratios of RNA isolated by the STE method ranged between 1.80～2.10 and between 1.90～2.30 respectively,whereas the A260/A280 ratio of RNA isolated by the Trizol method was below 1.80,and the A260/A280 ratio was outside the 1.90～2.30 *** distinct bands representing 28S,18S and 5S rRNA were observed following electrophoresis of total RNA isolated from the three test strains by the STE *** of total RNA isolated using the Trizol method resulted in dark bands corresponding to 28S rRNA and 18S rRNA,indicating partial degradation of the *** data demonstrated that the total RNA isolated by the STE method was of a quality eminently suited for downstream applications.