A CLOSE-CHEST METHOD FOR CREATING MYOCARDIAL ISCHEMIA AND REPERFUSION INJURY IN SWINE

作     者：韩蓓蓓 张昕 张庆勇 赵钢 魏均伯 马士新 魏盟 HAN Bei-bei, ZHANG Xin, ZHANG Qing-yong, ZHAO Gang, WEI Jun-bo, MA Shi-xin, WEI MengDepartment of Cardiology, Shanghai sixth People’s Hospital, Shanghai Jiaotong University, Shanghai 200233, China

作者机构：Department of Cardiology Shanghai sixth People’s Hospital Shanghai Jiaotong University 

出 版 物：《Journal of Shanghai Second Medical University(Foreign Language Edition)》 (上海第二医科大学学报（英文版）)

年 卷 期：2008年第20卷第1期

页      面：60-63,70页

学科分类：1002[医学-临床医学] 100201[医学-内科学(含：心血管病、血液病、呼吸系病、消化系病、内分泌与代谢病、肾病、风湿病、传染病)] 10[医学] 

主　　题：coronary artery occlusion myocardial infarction coronary artery catheterization 

摘      要：Objective To create a close-chest model of myocardial ischemia and reperfusion in pigs, using the technique of balloon catheter inflation and deflation, and to report experiences and pitfalls. MethodsIn 26 minipigs, a balloon catheter was advanced into the left descending coronary artery (LAD) via the femoral artery sheath and positioned at the midsection of LAD. LAD was occluded for 2 h and then reperfused. After 14 d, the animals were euthanized and the hearts were stained with triphenyltetrazolium chloride to identify infarct area. The causes for failure were analyzed. Results Seventeen pigs (65.38%) completed the experiment. The success rate increased from 52.94% in the initial 17 pigs to 88.89% in the last 9 pigs. The mean size of infarction was (16.4±4.1)% of the total left ventricular area. The direct cause for failure was lethal ventricular fibrillation (VF), with different underlying mechanisms according to different time of episode. Appropriate anticoagulation, well-controlled heart rate and proper size of balloon catheter were important factors for avoiding *** The technique of balloon inflation and deflation can be successfully used for creating the close-chest model of myocardial ischemia and reperfusion in pigs. This model is suitable for studies on myocardial infarction and early reperfusion, avoiding the disadvantages of open-chest models.