A simple method for inducing nonalcoholic steatohepatitis with fibrosis

作     者：Leyla Yahaghi Azadeh Ebrahim-Habibi Nasim Hayati-Roodbari Shiva Irani Parichehreh Yaghmaei 

作者机构：Department of BiologyScience and Research BranchIslamic Azad UniversityTehranIran Biosensor Research CenterEndocrinology and Metabolism Molecular-Cellular Sciences InstituteTehran University of Medical SciencesTehranIran Endocrinology and Metabolism Research CenterTehran University of Medical SciencesTehranIran 

出 版 物：《Animal Models and Experimental Medicine》 (动物模型与实验医学（英文）)

年 卷 期：2019年第2卷第4期

页      面：282-290页

核心收录：

学科分类：1002[医学-临床医学] 100201[医学-内科学(含：心血管病、血液病、呼吸系病、消化系病、内分泌与代谢病、肾病、风湿病、传染病)] 10[医学] 

基　　金：Mr Mohammad Fatemi Motlagh 

主　　题：animal model nonalcoholic fatty liver disease(NAFLD) nonalcoholic steatohepatitis(NASH) PPAR‐α 

摘      要：Background: Nonalcoholic fatty liver disease(NAFLD) is increasingly occurring in sedentary people, and may progress to NASH and hepatocellular carcinoma. It is essential to design affordable animal models for the study of various diseases, including fatty liver, which was the aim of the study. In this study, a high-fat diet was devised that triggers NASH’s animal model quickly and easily. High-fat diet(HFD) was used both with intra-mouth oral gavage and in combination with animal ***: Twenty-four male C57 BL/6 J mice were divided into HFD and ND groups, which received a high-fat diet and a normal diet, respectively. At the end of the experiment(fourth week of treatment), body and liver weights, biochemical parameters, PPAR-α gene expression and histopathologic characteristics of the liver were ***: During 4 weeks, body weight of mice did not show a significant increase in the HFD group compared to the ND group, while weight gain of the liver was significant. Histological assessment of the HFD group’s liver confirmed NASH symptoms. In the HFD group, HDL-c, SOD, catalase, FRAP, adiponectin, and PPAR-α decreased significantly, and lipid profiles, hepatic enzymes, MDA, leptin, and TNF-α showed a significant increase compared to the ND ***: Our high-fat diet has successfully induced all aspects of NASH with fibrosis in 4 weeks, and with low cost.