The Determination of Canthaxanthin in Feed Ingredients by High Performance Liquid Chromatography
The Determination of Canthaxanthin in Feed Ingredients by High Performance Liquid Chromatography作者机构:School of Food Science and Engineering Harbin Institute of Technology Harbin 150090 China
出 版 物:《Journal of Northeast Agricultural University(English Edition)》 (东北农业大学学报(英文版))
年 卷 期:2008年第15卷第2期
页 面:37-40页
学科分类:090502[农学-动物营养与饲料科学] 081704[工学-应用化学] 07[理学] 08[工学] 0817[工学-化学工程与技术] 0905[农学-畜牧学] 09[农学] 070302[理学-分析化学] 0703[理学-化学]
主 题:HPLC feed canthaxanthin carotenoids
摘 要:A series of studies were conducted to establish a methodology for the accurate and efficient determination of canthaxanthin in feed ingredients. Agilent ZORBAX Eclipse DB-C 18 analytical column (4.6×150 mm, 5μm) was used and kept at 25 ℃. The mobile phase was acetonitrile : methanol (v/v)=95 : 5, and eluted compounds were detected by DAD absorbance (470 nm). The flow rate was maintained at 1.0 mL·min^-1. The response of the method was linear over the range 1.0-20.0 μg·mL^-1 canthaxanthin assay solution (R^2=0.9998). Recovery assays done with standard canthaxanthin to feed ingredient resulted in an average recovery of 103%. Variation coefficient was less than 3.53%. This method is proved to be simple, precise, sensitive and reproductive.