In vitro anti-biofilm and anti-bacterial activity of Junceella juncea for its biomedical application

作     者：P Kumar S Senthamil Selvi M Govindaraju 

作者机构：Department of Environmental BiotechnologyBharathidasan University Department of Plant ScienceBharathidasan University 

出 版 物：《Asian Pacific Journal of Tropical Biomedicine》 (亚太热带生物医学杂志（英文版）)

年 卷 期：2012年第2卷第12期

页      面：930-935页

学科分类：0710[理学-生物学] 0831[工学-生物医学工程（可授工学、理学、医学学位）] 08[工学] 0836[工学-生物工程] 

基　　金：Supported by DST-NRDMS Government of India(grant No.041594/F3/2008/dt.08.12.2010) 

主　　题：Junceella juncea Anti-biofilm Anti-bacterial FT-IR Congo red agar Microtitre plate assay 

摘      要：Objective:To investigate the anti-biofilm and anti-bacterial activity of Junceella juncea(***)against biofilm forming pathogenic ***:Gorgonians were extraeted with methanol and analysed with fourier transform infrared *** forming pathogens were identified by Congo red agar supplemented with sucrose.A quantitative spectrophotometric method was used to monitor in vitro biofilm reduction by microtitre plate ***-bacterial activity of methanolic gorgonian extract(MGE)was carried out by disc diffusion method followed by calculating the percentage of increase with crude methanol(CM).Results:The presence of active functional group was exemplified by FT-IR ***,black,crystalline colonies confirm the production of extracellular polymeric substances responsible for biofilm formation in Congo red *** exhibited potential anti-biofilm activity against all tested bacterial *** anti-bacterial activity of methanolic extract was comparably higher in Salmonella typhii followed by Escherichia colt,Vibrio cholerae and Shigella *** overall percentage of increase was higher by 50.2%to ***:To conclude,anti-biofilm and anti-bacterial efficacy of *** is impressive over biofilm producing pathogens and are good source for novel anti-bacterial compounds.