Amomum villosum induces longitudinal bone growth in adolescent female rats

作     者：Sun Haeng Lee Ji Young Kim Hocheol Kim Seul Ki Park Cho Young Kim Sun Yong Chung Gyu Tae Chang 

作者机构：Department of Pediatrics of Korean MedicineKyung Hee University Hospital at Gang-dong892 Dongnam-roGangdong-guSeoul 134-727Republic of Korea Department of Herbal PharmacologyCollege of Korean MedicineKyung Hee University26 Kyungheedae-roDongdaemoon-guSeoul 130-701Republic of Korea Department of Neuropsychiatry of Korean MedicineKyung Hee University Hospital at Gang-dong892 Dongnam-roGangdong-guSeoul 134727Republic of Korea 

出 版 物：《Journal of Traditional Chinese Medicine》 (中医杂志（英文版）)

年 卷 期：2012年第32卷第3期

页      面：453-458页

核心收录：

学科分类：1008[医学-中药学(可授医学、理学学位)] 1007[医学-药学(可授医学、理学学位)] 1006[医学-中西医结合] 1005[医学-中医学] 1002[医学-临床医学] 100602[医学-中西医结合临床] 10[医学] 

基　　金：Supported by the Fund of Amomum villosum in bone growth 

主　　题：Amomum villosum Growth plate Bone Development Recombinant human growth hormone 

摘      要：OBJECTIVE:This study was conducted to evaluate the effect of Amomum villosum on longitudinal bone ***:Adolescent female Sprague-Dawley rats were divided into 3 groups and treated for 4 days:control(distilled water,p.o.),recombinant human growth hormone(rhGH;100 μg/kg,s.c.),and ***(500 mg/kg,p.o.) *** day 3,tetracycline(20 g/kg,i.p.) was injected for growth plate *** days 2,3 and 4,5-bromo-2 -deoxyuridine(BrdU)(50 mg/kg,i.p.) was injected to label proliferating *** day 5,tibias were dissected and fixed in 4% paraformaldehyde,dehydrated,and sectioned for immunohistochemistry and ***:The rate of bone growth in the *** and rhGH groups increased to(410 ± 44) and(389 ± 46) μm/day(P0.01),respectively,as compared with the control(330.7 ± 34.7) μm/*** thickness of the growth plates also increased to(591 ± 37) and(598 ± 32) μm,respectively,as compared with the control(524± 89) μm(P0.001).The number of BrdU-positive cells in the chondrocytes of the *** and rhGH groups was also significantly higher(126±24) and(143±18) cells/mm 2,respectively) than in the control(109 ± 25) mm 2(P0.05).Insulin-like growth factor-1 and bone morphogenetic protein-2 in the *** and rhGH groups were highly expressed in the growth plate as compared with the control samples,indicating increased bone ***:*** could be used to treat growth retardation during adolescence.