Chromosome 14q may harbor multiple tumor suppressor genes in primary glioblastoma multiforme

作     者：胡杰 江澄川 吴浩强 彭颂先 唐婉君 HU Jie;JIANG Chengchuan;Ho-Keung Ng;Jesse CS Pang;Carol YK Tong

作者机构：复旦大学附属华山医院神经外科上海200040 香港中文大学病理解剖及细胞学系 

出 版 物：《Chinese Medical Journal》 (中华医学杂志（英文版）)

年 卷 期：2002年第115卷第8期

页      面：1201-1204页

核心收录：

学科分类：1002[医学-临床医学] 100214[医学-肿瘤学] 10[医学] 

主　　题：Chromosomes, Human, Pair 14 Genes, Tumor Suppressor Loss of Heterozygosity Adult Aged Female Glioblastoma Humans Male Microsatellite Repeats Middle Aged 

摘      要：OBJECTIVE: To evaluate whether deletion of chromosome 14q is involved in the carcinogenesis of primary glioblastoma multiforme and to identify possibly common deletion regions. METHJODS: Fourteen fluorescent dye-labeled polymorphic markers were used and polymerase chain reaction-based microsatellite analysis was employed to investigate loss of heterozygosity (LOH) on chromosome 14q in 20 primary glioblastoma multiforme (GBM). RESULTS: Ten of twenty (50%) GBM displayed LOH at one or more of the markers on chromosome 14q. Five tumors showed either LOH or non-informative on all markers tested. The most frequent LOH was observed at locus D14S65 (57.1%) on 14q32.1, and in the chromosomal region spanning from D14S63 (47.1%) to D14S74 (46.7%) on 14q23-31. None of the informative loci exhibited microsatellite instability. CONCLUSIONS: Allelic deletion on chromosome 14q plays an important role in the pathogenesis of GBM. Chromosomal regions at locus D14S65 on 14q32.1 and spanning from D14S63 to D14S74 on 14q23-31 may harbor multiple tumor suppressor genes associated with GBM.