Comparisons in subcellular and biochemical behaviors of cadmium between Iow-Cd and high-Cd accumulation cultivars of pakchoi （Brassica chinensis L.）

作     者：Meng XUE Yihui ZHOU Zhongyi YANG Biyun LIN Jiangang YUAN Shanshan WU 

作者机构：State Key Laboratory for Biocontrol School of Life Sciences Sun Yat-Sen University Guangzhou 510275 China 

出 版 物：《Frontiers of Environmental Science & Engineering》 (环境科学与工程前沿（英文）)

年 卷 期：2014年第8卷第2期

页      面：226-238页

核心收录：

学科分类：0830[工学-环境科学与工程（可授工学、理学、农学学位）] 07[理学] 09[农学] 0903[农学-农业资源与环境] 0901[农学-作物学] 0713[理学-生态学] 

基　　金：国家自然科学基金 Key Research Project of Guangdong Province State Key Project for Science and Technology Development of China 

主　　题：cadmium (Cd) low-Cd cultivar pakchoi (Brassica chinensis L.) subcellular distribution chemical forms 

摘      要：Subcellular distributions and chemical forms of cadmium （Cd） in the leaves, stems and roots were investigated in low-Cd accumulation cultivars and high-Cd accumulation cultivars ofpakchoi （Brassica chinensis L.）. Root cell wall played a key role in limiting soil Cd from entering the protoplast, especially in the low-Cd cultivars. The high-Cd cultivars had significantly higher leaf and stem Cd concentrations than the low-Cd cultivars in cell wall fraction, chloroplast/trophoplast fraction, organelle fraction and soluble fraction. In low-Cd cultivars, which were more sensitive and thus had greater physiological needs of Cd detoxification than high-Cd cultivars, leaf vacuole sequestrated higher proportions of Cd. Cd in the form of pectate/protein complexes （extracted by 1 tool. L~ NaC1） played a decisive role in Cd translocation from root to shoot, which might be one of the mechanisms that led to the differences in shoot Cd accumulation between the two types of cultivars. Furthermore, the formation of Cd- phosphate complexes （extracted by 2% HAc） was also involved in Cd detoxification within the roots of pakchoi under high Cd stress, suggesting that the mechanisms of Cd detoxification might be different between low- and high-Cd cultivars.