Prenatal express-diagnosis by the method of QF-PCR and automatic microelectroforesis with microarrays

作     者：Zaporozhan VN Bubnov VV Marichereda VG Verbitskaya TG Belous OB Zaporozhan VN;Bubnov VV;Marichereda VG;Verbitskaya TG;Belous OB

作者机构：Odessa National Medical University 

出 版 物：《中国现代医学杂志》 (China Journal of Modern Medicine)

年 卷 期：2011年第21卷第27期

页      面：3346-3348页

学科分类：1004[医学-公共卫生与预防医学(可授医学、理学学位)] 10[医学] 

主　　题：医生 诊断 临床 QF-PCR 

摘      要：The modern molecular-genetic methods have been implementing actively into the medical practice.They improve diagnostic accuracy,help to prognosticate the course of oncological diseases,optimize the results of prenatal diagnosis,decrease mothers anxiety and improve the clinical outcomes of pregnancy.There are used the various traditional approaches e.***.cariotyping,FISH and more contemporary-real-time PCR,comparative genomic hybridization(CGH) or chromosomal microarray analysis(CMA),Quantitative Fluorescent PCR(QF-PCR).For expressing diagnosis of triploidy by 21st and 18th chromosomes there was used QF-PCR technologies with the consequent quantative analysis on the automatic capillary microelectrophoresis on the microarrays Expеrion DNA1K.There was determined that diagnostic accuracy of QF-PCR was comparable with existing routine methods,but it had some advantages including expressity and could be recommended for implementation into practical medicine.