Polypeptide from Chlamys farreri inhibits UVB-induced apoptosis of HaCaT cells via iNOS/NO and HSP90

作     者：张正洋 刘小金 刘拓 闫琳 王跃军 王春波 

作者机构：Department of PharmacologyMedical College of Qingdao University Department of Public HealthMedical College of Qingdao University Yellow Sea Fisheries Research InstituteChinese Academy of Fishery Sciences 

出 版 物：《Chinese Journal of Oceanology and Limnology》 (中国海洋湖沼学报（英文版）)

年 卷 期：2009年第27卷第3期

页      面：594-599页

核心收录：

学科分类：0908[农学-水产] 09[农学] 

基　　金：Supported by the National Natural Science Foundation of China (30471458) National Natural Science Foundation of Shandong Province (Z2007c09) 

主　　题：apoptosis HaCaT cells HSP iNOS/NO Polypeptide from Chlamysfarreri (PCF) ultraviolet B (UVB) 

摘      要：Polypeptide from Chlamys farreri(PCF) is a novel marine bioactive product that was isolated from the gonochoric Chinese scallop Chlamys farreri,and was found to be an effective antioxidant in our recent *** this study,we investigated the effect of PCF on ultraviolet B(UVB)-induced apoptosis of HaCaT cells and the intracellular signaling pathways *** with the inducible nitric oxide synthase(iNOS) inhibitor S-methylisothiourea sulfate inhibited UVB-induced apoptosis,indicating that iNOS and NO play important roles in *** the other hand,the inhibition of UVB-induced apoptosis in the immortalized keratinocyte(HaCaT) cells by PCF was estimated using a DNA *** treatment inhibited UVB-induced iNOS activation,as determined by RT-PCR,NO production,as determined by ESR,and up-regulated heat shock protein(HSP) 90 activation,as determined by Western *** results indicate that iNOS and NO are involved in UVB-induced apoptosis of HaCaT cells and the protective effect of PCF against UVB irradiation is exerted by suppressing the expression of iNOS,followed by inhibition of NO release and enhanced activation of HSP90.