Peak compression technique in high-performance liquid chromatography

作     者：WEI YuXia WANG Lin XIAO ShengYuan QING Hong ZHU Yong HU GaoFei DENG YuLin 

作者机构：School of Life Science and Technology Beijing Institute of Technology Beijing 100081 China 

出 版 物：《Chinese Science Bulletin》 (CHINESE SCIENCE BULLETIN)

年 卷 期：2009年第54卷第16期

页      面：2805-2809页

核心收录：

学科分类：0810[工学-信息与通信工程] 081704[工学-应用化学] 07[理学] 08[工学] 0817[工学-化学工程与技术] 070302[理学-分析化学] 081001[工学-通信与信息系统] 0703[理学-化学] 

基　　金：Supported by the National Key Technology R&D Program in the 11th Five-year Plan (Grant No. 2007CB914100) 

主　　题：压缩技术 高效液相色谱 模型化合物 脲甲醛 迁移速度 羟基喹啉 压缩条件 运行周期 

摘      要：Peak compression technique based on the difference of the solute migration velocity in two different mobile phases was described theoretically and confirmed using benzaldehyde and 4-hydroxyquinoline (4-HQ) as model compounds. After peak compression, the peak compression factors (the ratio of peak width at half-height under non-compression and that under compression condition) of benzaldehyde and 4-HQ were 0.19 and 0.13, respectively. By this application of the peak compression technique to the mixture, both enhanced peak height and good separation were obtained in one run cycle. This peak compression technique was introduced to determine benzaldehyde from semicarbazide-sensitive amine oxidase-catalyzed enzymetic reaction in order to illustrate the applicability of this technique to the real sample. As a result, the peak was compressed effectively, and 4.94-fold, 19.3-fold and 5.74-fold enhancement in peak height, plate number and signal to noise ratio were also achieved, respectively.