Self-assembled sialyllactosyl probes with aggregation-enhanced properties for ratiometric detection and blocking of influenza viruses

作     者：Wei-Tao Dou Zhao-Yang Qin Jun Li Dong-Ming Zhou Xiao-Peng He 

作者机构：Key Laboratory for Advanced MaterialsFeringa Nobel Prize Scientist Joint Research CenterSchool of Chernistry and Molecular EngineeringEast China University of Science and TechnologyShanghai 200237China Vaccine Research CenterKey Laboratory of Molecular Virology&ImmunologyInstitut Pasteur of ShanghaiChinese Acadermy of SciencesShanghai 200031China 

出 版 物：《Science Bulletin》 (科学通报（英文版）)

年 卷 期：2019年第64卷第24期

页      面：1902-1909页

核心收录：

学科分类：1002[医学-临床医学] 100201[医学-内科学(含：心血管病、血液病、呼吸系病、消化系病、内分泌与代谢病、肾病、风湿病、传染病)] 10[医学] 

基　　金：supported by the National Natural Science Foundation of China (21788102,91853201,21722801,21776078) the Shanghai Municipal Science and Technology Major Project (2018SHZDZX03) the National Postdoctoral Program for Innovative Talents (BX20190115) 

主　　题：Vibration-induced emission Influenza viruses Ratiometric Fluorescence Glycoprobe 

摘      要：Infection and dissemination of influenza viruses(IVs) causes serious health concerns ***, effective tools for the accurate detection and blocking of IVs remain elusive. Here, we develop a new sialyllactosyl probe with self-assembled core-shell structure for the ratiometric detection and blocking of IVs. N,N -diaryl-dihydrodibenzo[a,c]phenazines were used to form the core structure by hydrophobic assembly in an aqueous solution with an aggregation-enhanced blue fluorescence ***, dicyanomethylene-4 H-pyran-based sialyllactosides were used for self-assembly with the core structure, producing the sialyllactosyl probe that emits a red fluorescence due to Forster resonance energy transfer. The probe developed has been proven to be available for(1) the fluorescence ratiometric detection of IVs through selective interaction with the sialyllactosyl-binding proteins on the virus surface,and(2) effectively blocking the invasion of human-infecting IVs towards host cells as accentuated by the sialyllactosides on the surface of the probes.