Design of a 1,8-naphthalimide-based OFF-ON type bioorthogonal reagent for fluorescent imaging in live cells

作     者：Zhuzhou Shao Chun Zhang Xiaohua Zhu Yajun Wang Wenyuan Xu Yu Chen Xiaoming Wang Hailiang Zhu Yong Liang 

作者机构：State Key Laboratory of Coordination ChemistryJiangsu Key Laboratory of Advanced Organic MaterialsSchool of Chemistry and Chemical EngineeringNanjing UniversityNanjing 210023China State Key Laboratory of Pharmaceutical BiotechnologySchool of Life SciencesNanjing UniversityNanjing 210023China 

出 版 物：《Chinese Chemical Letters》 (中国化学快报（英文版）)

年 卷 期：2019年第30卷第12期

页      面：2169-2172页

核心收录：

学科分类：0710[理学-生物学] 081704[工学-应用化学] 07[理学] 08[工学] 0817[工学-化学工程与技术] 09[农学] 070302[理学-分析化学] 0703[理学-化学] 

基　　金：financially supported by the National Natural Science Foundation of China (No.21803030) the National Thousand Young Talents Program the Jiangsu Specially-Appointed Professor Plan the NSF of Jiangsu Province (No.BK20170631) in China 

主　　题：Bioorthogonal cycloaddition 1,8-Naphthalimide Photophysical property Sydnone Turn-on fluorescence 

摘      要：A novel 1,8-naphthalimide-based OFF-ON type fluorogenic sydnone(Naph-Syd) is designed as bioorthogonal probe for *** moiety efficiently quenches the native fluorescence of 1,8-naphthalimide,which can be restored with the enhancement of about 300-fold,after reacting with strained cyclooctynes to form pyrazole products(Naph-Pyr).The second-order rate constant of this bioorthogonal cycloaddition can be up to 2.5 L mol^-1s^-1,which benefits imaging of biomolecules at low concentrations in cellular environment.