Simultaneous Determination of Four Ingredients in Guanxin Danshen Tablets by RP-HPLC

作     者：Yougen WU Guihua ZENG Daoan XIAO Yujun WU Yanjun CHEN Juan LIU 

作者机构：Nanchang Vocational UniversityNanchang 330050China Nanchang County Hospital of Traditional Chinese MedicineNanchang 330202China Institute of Chemistry and Biological EngineeringYichun UniversityYichun 336000China Jiangxi Normal UniversityNanchang 330004China 

出 版 物：《Medicinal Plant》 (药用植物：英文版)

年 卷 期：2019年第10卷第5期

页      面：18-19,27页

学科分类：1008[医学-中药学(可授医学、理学学位)] 1006[医学-中西医结合] 100602[医学-中西医结合临床] 10[医学] 

基　　金：Supported by Science and Technology Research Project of Jiangxi Provincial Department of Education(GJJ181381) 

主　　题：HPLC Simultaneous determination Guanxin Danshen tablets 

摘      要：[Objectives]This study aimed to establish a method for simultaneous determination of four ingredients in Guanxin Danshen tablets by RP-HPLC.[Methods]HPLC chromatography was adopted with column of Thermo Hypersil-Keystone C 18 column(4.6 mm×250 mm,5μm),mobile phase of acetonitrile(A)-0.03 mol/L ammonium acetate(pH adjusted to 2.4 with formic acid)(B),gradient elution(0-5 min,5%A;5-10 min,5%A→19%A;10-40 min,19%A;40-68 min,19%A→36%A;68-90 min,36%A→95%A),flow rate of 1.0 mL/min and column temperature of 25℃.[Results]The content of salvianic acid A sodium,protocatechuic aldehyde,salvianolic acid B and tanshinone II A showed good linear relationship with chromatographic peak area in the range of 3.310-18.66,0.03950-0.2370,0.7500-4.500,0.05920-0.3550μg,*** recovery rate(n=6)was 101.75%,96.86%,104.15%and 99.03%,respectively,and the RSD was 1.52%,2.81%,1.80%,and 1.37%*** established method has good precision,reproducibility and stability.[Conclusions]This method can be used for the quality control of multiple ingredients of Guanxin Danshen tablets.