FITC labeling of human insulin and transport of FITC-insulin conjugates through MDCK cell monolayer

作     者：Darshana Shah Yuxing Guo Joseph Ocando Jun Shao 

作者机构：Avion Pharmaceuticals1880 McFarland ParkwaySuite 110-2Alpharett1G130005USA Department of Pharmaceutical SciencesCollege of Pharmacy and Health SciencesSt.John's University8000 Utopia ParkwayQueensNY11439USA 

出 版 物：《Journal of Pharmaceutical Analysis》 (药物分析学报（英文版）)

年 卷 期：2019年第9卷第6期

页      面：400-405页

核心收录：

学科分类：1002[医学-临床医学] 100201[医学-内科学(含：心血管病、血液病、呼吸系病、消化系病、内分泌与代谢病、肾病、风湿病、传染病)] 10[医学] 

基　　金：St. John's University  SJU 

主　　题：Insulin FITC labeling Conjugates MDCK cells Mono-conjugate Tri-conjugate 

摘      要：Fluorescein isothiocyanate-labeled insulin(FITC-insulin)has been widely used for bioanalytical *** to the high cost of commercial FITC-insulin and tedious labeling procedures described in the literature,there is still a need to develop a cost effective,reliable and quick labeling method for *** purpose of the present work was to develop a quick and affordable method for FITC labeling of human insulin and to determine the effect of different conjugations of FITC to human insulin on its permeability through the MDCK cell *** labeling of insulin gives mono-,di-or tri-conjugates depending on the reaction time and the molar ratio of FITC:***-conjugate with unlabeled insulin,mixture of di-and tri-conjugate,and tri-conjugate with very little amount of di-conjugate were synthesized in less than 4 *** of conjugation had an effect on the permeability of insulin through the MDCK cell ***-conjugate had higher permeability than the unlabeled insulin due to increase in partition ***,tri-conjugate showed lower permeability than the unlabeled insulin due to the increase in molecular weight.