Preparation and Charaterization of Self-assembled Nanoparticles Based on Linolenic-acid Modified Chitosan

作     者：LIU Chenguang Desai Kashappa Goud H. CHEN Xiguang Park Hyun-Jin 

作者机构：College of Life Science Ocean University of China Qingdao 266003 P.R. China Graduate School of Biotechnology Korea University Seoul 136-701 Korea 

出 版 物：《Journal of Ocean University of China》 (中国海洋大学学报（英文版）)

年 卷 期：2005年第4卷第3期

页      面：234-239页

学科分类：081704[工学-应用化学] 07[理学] 08[工学] 0817[工学-化学工程与技术] 070303[理学-有机化学] 0703[理学-化学] 

基　　金：National Natural Science Foundation of China(30370344) Korea Science and Engineering Foundation(19992-220-009-4)supported this study 

主　　题：chitosan linolenic acid nanoparticles self-aggregates BSA 

摘      要：Chitosan was modified by conjugating coupling with linolenic acid through the 1-ethyl-3-（3-dimethylami- nopropyyl） carbodiimide （EDC）-mediated reaction. The degree of substitution 1.8% （ i.e. 1.8 linolenic acid group per 100 anhydroglucose units） was measured by ^1H NMR. The critical aggregation concentration （CAC） of the self-aggregate of hydrophobically modified chitosan was determined by measuring the fluorescence intensity of the pyrene as a fluorescent probe. The CAC value in phosphate-buffered saline （PBS） solution （pH 7.4） was 5 × 10^-2 mg mL^-1. The average particle size of selfaggregates of hydrophobically modified chitosan in PBS solution （pH7.4） was 210.8 nm with a unimodal size distribution ranging from 100 to 500 nm. Transmission electron microscopy （TEM） study showed that the formation of near spherical shape nanoparticles has enough structural integrity. The loading ability of hydrophibically modified chitosan （LA-chitosan） was investigated by using bovine serum albumin （BSA） as the model. The loading capacity of self-aggregated nanoparticles increases （ 19.85 % ± 0.04 % to 37.57 % ± 0.25 % ） with the concentration of BSA （0.1-0.5 mg mL^-1 ）.