Aryl hydrocarbon receptor expression in serum,peripheral blood mononuclear cells,and skin lesions of patients with atopic dermatitis and its correlation with disease severity

作     者：Yu-Qing Hu Ping Liu Zhang-Lei Mu Jian-Zhong Zhang Yu-Qing Hu;Ping Liu;Zhang-Lei Mu;Jian-Zhong Zhang

作者机构：Department of DermatologyPeking University People’s HospitalBeijing 100044China 

出 版 物：《Chinese Medical Journal》 (中华医学杂志（英文版）)

年 卷 期：2020年第133卷第2期

页      面：148-153页

核心收录：

学科分类：1002[医学-临床医学] 100206[医学-皮肤病与性病学] 10[医学] 

主　　题：Aryl hydrocarbon receptor Cytochrome P450 Atopic dermatitis Peripheral blood mononuclear 

摘      要：Background:The aryl hydrocarbon receptor(AhR)is a ligand-activated transcription factor,which is critically involved in the pathogenesis of a variety of skin *** aim of this study was to detect AhR and its downstream regulators including cytochrome P450(CYP1A1),AhR nuclear translocation(ARNT),and aryl hydrocarbon receptor repressor(AhRR)in serum,peripheral blood mononuclear cells(PBMCs),and skin lesions in patients with atopic dermatitis(AD).Methods:Twenty-nine AD patients defined according to the criteria of Hanifin and Rajka and Chinese criteria of AD were *** without allergic and chronic diseases were recruited as *** and controls were selected from the dermatology outpatient clinic of Peking University People’s Hospital from August 1 to December 31 in ***-linked immunosorbent assay was performed to detect serum AhR *** of AhR,AhRR,ARNT,and CYP1A1 in PBMCs were measured by realtime quantitative polymerase chain *** expression in skin lesions was measured by ***:AhR was significantly higher expressed in serum(41.26±4.52 vs.33.73±2.49 pmol/L,t=6.507,P0.001)and skin lesions(0.191±0.041 vs.0.087±0.017,t=10.036,P0.001)of AD patients compared with those of *** mRNA levels of AhR(1.572±0.392 vs.1.000±0.173,t=6.819,P0.001),AhRR(2.402±1.716 vs.1.000±0.788,t=3.722,P0.001),CYP1A1(2.258±1.598 vs.1.000±0.796,t=3.400,P=0.002)in PBMCs of AD patients were higher compared with those of *** difference in mRNA levels of ARNT was not statistically significant between the patients and controls(1.383±0.842 vs.1.000±0.586,t=1.653,P=0.105).AhR mRNA levels in PBMCs positively correlated with eczema area and severity index score and serum interleukin-6 ***:AhR and its downstream regulators were highly expressed in serum,PBMCs,and skin of AD patients,which might contribute to the pathogenesis of AD.