Staphylococcus aureusβ-hemolysin-neutralizing single-domain antibody isolated from phage display library of Indian desert camel

作     者：Jangra Pooja Singh Ajit 

作者机构：Immunology SectionDepartment of Veterinary MicrobiologyCh.Charan Singh Haryana Agricultural University 

出 版 物：《Asian Pacific Journal of Tropical Medicine》 (亚太热带医药杂志（英文版）)

年 卷 期：2010年第3卷第1期

页      面：1-7页

核心收录：

学科分类：1010[医学-医学技术（可授医学、理学学位）] 10[医学] 

基　　金：Financial support by Department of Biotechnology Government of India New Delhi for construction of phage display library and its applications  and Indian Council of Agricultural Research New Delhi for infrastructure assistance 

主　　题：Staphylococcus aureus β-hemolysin neutralization Single domain antibodies Phage display library Indian desert camel 

摘      要：Objective:To isolate and characterize Staphylococcus aureus(***)β-hemolysinneutralizing dAbs from phage display library of Indian desert ***:Phage display library of 5×10 dAb clones of LPS-immunized Indian desert camel constructed in our laboratory was used for selection of *** exotoxin-specific clones by panning *** of Ag-specific clones in successive rounds of panning was assessed by phage-ELISA and phage *** dAb clones binding to *** exotoxin Ags were expressed with C-terminal 6×His tag in *** and purified by Ni-chelate *** expression was verified by SDS-PAGE and western *** purified clones were tested for inhibition of ’hot-cold’ hemolytic activity in *** to thermal inactivation of the dAb clones was studied by observing the effect of heat treatment from 50℃to 99℃for 30 min on the ’hot-cold’ hemolytic activity in ***:Several dAb clones binding to *** exotoxins were isolated and enriched by three rounds of *** soluble dAb clones were approximately～16 kDa in size and reacted with 6×His tag specific murine monoclonal antibody in western *** of the Ni-chelate affinity purified dAb.6×His clones,inhibited ***β-hemolysin activity in vitro and resisted thermal inactivation upto ***:An ***β-hemolysinneutralizing dAb clone of possible therapeutic potential has been isolated.