Effects of Quercetin on Hedgehog Signaling in Chronic Myeloid Leukemia KBM7 Cells

作     者：李蔚 赵瑛 陶波 张颖 

作者机构：Department of PharmacyHarbin Commercial University 

出 版 物：《Chinese Journal of Integrative Medicine》 (中国结合医学杂志（英文版）)

年 卷 期：2014年第20卷第10期

页      面：776-781页

核心收录：

学科分类：1008[医学-中药学(可授医学、理学学位)] 1007[医学-药学(可授医学、理学学位)] 1006[医学-中西医结合] 1005[医学-中医学] 1002[医学-临床医学] 100602[医学-中西医结合临床] 10[医学] 

基　　金：Supported by grants of Heilongjiang Health Bureau Technology Project(No.2011-520) Harbin Technology Research Fund(No.2012RFQYS073) 

主　　题：quercetin hedgehog signaling chronic myeloid leukemia apoptosis cell cycle Bcr-abl 

摘      要：Objective：To investigate the effects of quercetin on Hedgehog（Hh） signaling in chronic myeloid leukemia KBM7 ***：The KBM7 cells were treated with 50,100 and 200 μmol/L quercetin for48 h *** then the trypan blue assay was used to examine the proliferative inhibition of *** cells and cell cycle were measured by flow *** mRNA and protein expression were detected by quantitative real-time polymerase chain reaction（PCR） and Western blot,***：Quercetin significantly inhibited KBM7 cell proliferation,induced cell apoptosis,and blocked cell cycle at G1 phase,which were in dose-dependent *** mRNA and protein expression of Smoothened and Gliomal（Gli1）,the members of Hh pathway decreased after treatment with *** Bcl-2 and Cyclin D1,targets of Hh signaling,also decreased after treatment with quercetin,*** also could increase p53 and Caspase-3 ***-abl mRNA copies decreased,but no changes of phosphorylated Bcr-abl and Bcr-abl proteins were observed,after treatment with ***：Quercetin could inhibit Hh signaling and its downstream targets in the KBM7 *** it might be one of mechanisms of inducing apoptosis and inhibiting cell cycle by quercetin.