Apoptosis of Alveolar Wall Cells in Chronic Obstructive Pulmonary Disease Patients with Pulmonary Emphysema Is Involved in Emphysematous Changes

作     者：刘红梅 马利军 吴纪珍 王凯 陈献亮 

作者机构：Division of Respiratory DiseasesDepartment of Internal MedicineHe'nan Provincial People's Hospital 

出 版 物：《Journal of Huazhong University of Science and Technology(Medical Sciences)》 (华中科技大学学报（医学英德文版）)

年 卷 期：2009年第29卷第4期

页      面：466-469页

核心收录：

学科分类：0831[工学-生物医学工程（可授工学、理学、医学学位）] 0710[理学-生物学] 1002[医学-临床医学] 1001[医学-基础医学(可授医学、理学学位)] 100201[医学-内科学(含：心血管病、血液病、呼吸系病、消化系病、内分泌与代谢病、肾病、风湿病、传染病)] 0805[工学-材料科学与工程（可授工学、理学学位）] 0703[理学-化学] 0836[工学-生物工程] 10[医学] 

基　　金：supported by a program of medical research of He nan Province(No.200902012) 

主　　题：emphysema apoptosis proliferation 

摘      要：This study explored the role of apoptosis of alveolar wall cells of chronic obstructive pulmonary disease （COPD） patients with pulmonary emphysema in the pathogenesis of emphysema. The subjects were divided into three groups： COPD patients with pulmonary emphysema （COPD group）, asymptomatic smokers and non-smokers. Lung tissues were harvested and histologically assessed. TUNEL assay was employed to determine the ap＇optotic cells. The expression of PCNA, Bax and SP-C in the lung alveolar wall cells were immunohistochemically determined. SP-C immunofluorescence staining was used to identify type Ⅱ alveolar cells in the TUNEL-positive cells. The mean linear interval （MLI）, mean alveoli number （MAN） and mean alveoli area （MAA） in COPD group were significantly different as compared with those in asymptomatic smokers and non-smokers, respectively （P〈0.01）. The proliferation index （PI）, apoptosis index （AI） and the percentage of Bax-positive cells in COPD group were significantly greater than those of asymptomatic smokers and non-smokers （P〈0.01）. However, the percentage of SP-C-positive cells was significantly lower in COPD group than in asymptomatic smokers and non-smokers （P〈0.01）. Most of the TUNEL-positive cells expressed SP-C. In COPD group, the apoptosis of alveolar wall cells, especially apoptosis of type- Ⅱ cells, may take part in the pathogenesis of emphysema. Up-regulation of Bax expression may be responsible for the apoptosis of alveolar wall cells in the COPD patients with pulmonary emphysema.