Autophagy is increased in cryptorchid testis resulting in abnormal spermatozoa

作     者：Marina G Yefimova Antoine Buschiazzo Agnes Burel Marie-Therese Lavault Celine Pimentel Guilhem Jouve Sylvie Jaillard Bernard Jegou Nicolas Bourmeyster Celia Ravel 

作者机构：Univ RennesCHU RennesLaboratoire de Biologie de la Reproduction-CECOSInsermEH ESPIrset(Institut de recherche en santeenvironnement et travail)-UMR_S 1085Rennes F-35000France Sechenov Institute of Evolutionary Physiology and BiochemistryRussian Academy of Sciences194223 St-PetersburgRussia Univ RennesBiosit Platform-MRICRennes 35000France Univ RennesCHU RennesDepartment of Gynecology Obstetric and Human ReproductionRennes 35000France Univ RennesCHU RennesDepartment of CytogeneticIrset(Institute for Environmental and Occupational Health)-UMR_S 1085Rennes 35000France Univ RennesInsermEH ESPIrset(Institute for Environmental and Occupational Health)-UMR_S 1085Rennes F-35000France University of Poitiers-CHU PoitiersSTIM ERL 7003 CNRSPoitiers 86021France 

出 版 物：《Asian Journal of Andrology》 (亚洲男性学杂志（英文版）)

年 卷 期：2019年第21卷第6期

页      面：570-576页

核心收录：

学科分类：1002[医学-临床医学] 100214[医学-肿瘤学] 10[医学] 

基　　金：Agence de BioMedecine CHU de Rennes Rennes University Diabetes Research Center, DRC Institut National de la Santé et de la Recherche Médicale, Inserm 

主　　题：autophagy cryptorchidism mitophagy spermatozoa 

摘      要：Autophagy is invoIved in spermatogenesis by regulating germ cell maturation.This catabolic process increases with hyperthermic conditions to prevent the accumulation of damaged organelles.Cryptorchidism is associated with impairment of germ cell maturation revealed by the presence of immature forms of sperm cells in ejaculates.The aim of the present study was to evaluate the status of autophagy in sperm cells from cryptorchid patients.Semen samples of cryptorchid patients and normozoospermic controls were analyzed by immuno cytochemistry and electron microscopy.Autophagy protei ns,autophagy-related protein 9(ATG9)and microtubule-associated protein,lA/lB-light chain 3(LC3)were localized by immunocytochemistry on the acrosome and on the equatorial segment of sperm cells.LC3 was also detected in the midpiece of cryptorchid sperm tail.Autophagy substrate p62 protein was present in the acrosome and in the postequatorial segment of sperm in control samples,but not in the cryptorchid ones.Transmission electron microscopy revealed double-membrane-limited autophagosomes in postequatorial part of spermatozoa head and midpiece in cryptorchid samples.Partly degraded mitochondria were frequently discerned in autophagic vacuoles.In conclusion,autophagy is increased in sperm cells from patients with cryptorchid history comparatively to control.Our work provides insights into the role of autophagy in the maturation and survival of human male gametes in pathological conditions.Thus,regulating autophagy could represent a potential way to improve sperm quality in cryptorchid men.