Human natural killer cells for targeting delivery of gold nanostars and bimodal imaging directed photothermal/photodynamic therapy and immunotherapy

作     者：Bin Liu Wen Cao Jin Cheng Sisi Fan Shaojun Pan Lirui Wang Jiaqi Niu Yunxiang Pan Yanlei Liu Xiyang Sun Lijun Ma Jie Song Jian Ni Daxiang Cui 

作者机构：Institute of Nano Biomedicine and EngineeringShanghai Engineering Research Centre for Intelligent Diagnosis and Treatment InstrumentDepartment of Instrument Science and EngineeringSchool of Electronic Information and Electrical EngineeringShanghai Jiao Tong UniversityShanghai 200240China National Center for Translational MedicineCollaborative Innovational Center for System BiologyShanghai Jiao Tong UniversityShanghai 200240China Bio-X InstitutesKey Laboratory for the Genetics of Developmental and Neuropsychiatric Disorders(Ministry of Education)Shanghai Jiao Tong UniversityShanghai 200030China School of Biomedical EngineeringShanghai Jiao Tong UniversityShanghai 200240China Department of OncologyTongren HospitalShanghai Jiao Tong University School of MedicineShanghai 200336China 

出 版 物：《Cancer Biology & Medicine》 (癌症生物学与医学（英文版）)

年 卷 期：2019年第16卷第4期

页      面：756-770页

核心收录：

学科分类：1002[医学-临床医学] 100214[医学-肿瘤学] 10[医学] 

基　　金：supported from 973 Project (Grant No. 2015CB931802 and 2017YFA0205301) Chinese National Natural Scientific Fund (Grant No.81327002 and 81803094) China Postdoctoral Science Foundation (Grant No. 2017M621486) Funding from Shanghai Engineering Research Center for Intelligent diagnosis and treatment instrument (Grant No.15DZ2252000) 

主　　题：Gold nanostars natural killer cells photothermal therapy photodynamic therapy immunotherapy 

摘      要：Objective:To construct a novel nanoplatform GNS@CaCO3/Ce6-NK by loading the CaCO3-coated gold nanostars(GNSs)with Chlorin e6 molecules(Ce6)into human peripheral blood mononuclear cells(PBMCs)-derived NK cells for tumor targeted ***:GNS@CaCO3/Ce6 nanoparticles were prepared and characterized by TEM and *** cell surface markers and cytokines secretion of NK cells before and after loading the GNS@CaCO3/Ce6 nanoparticles were detected by Flow Cytometry(FCM)and *** of the GNS@CaCO3/Ce6-NK cells on A549 cancer cells was determined by FCM and *** fluorescent signals of GNS@CaCO3/Ce6-NK cells were detected via Confocal laser scanning microscopic(CLSM)and FCM at different time *** ROS generation of GNS@CaCO3/Ce6-NK cells under laser irradiation were examined by *** distribution of GNS@CaCO3/Ce6-NK in A549 tumor-bearing mice were observed by fluorescence imaging and PA *** combination therapy of GNS@CaCO3/Ce6-NK under laser irradiation were investigated on tumor-bearing ***:The coated CaC03 shell on the surface of GNSs exhibited prominent delivery and protection effect of Ce6 during the cellular uptake *** as-prepared multifunctional GNS@CaCO3/Ce6-NK cells possessed bimodal functions of fluorescence imaging and photoacoustic *** as-prepared multifunctional GNS@CaCO3/Ce6-NK cells could actively target tumor tissues with the enhanced photothermal/photodynamic therapy and ***:The GNS@CaCO3/Ce6-NK shows effective tumor-targeting ability and prominent therapeutic efficacy toward lung cancer A549 tumor-bearing *** fully utilizing the features of GNSs and NK cells,this new nanoplatform provides a new synergistic strategy for enhanced photothermal/photodynamic therapy and immunotherapy in the field of anticancer development in the near future.