Quantitative phase imaging of erythrocytes under microfluidic constriction in a high refractive index medium reveals water content changes
作者机构：Department of Biomedical EngineeringDuke UniversityDurhamNC 27708USA Department of Molecular Genetics and MicrobiologyDuke UniversityDurhamNC 27708USA Duke Center for Genomic and Computational BiologyDuke UniversityDurhamNC 27708USA Department of BiochemistryDuke UniversityDurhamNC 27708USA Department of Pathology and Laboratory MedicineEmory University School of MedicineAtlantaGA 30322USA
出 版 物：《Microsystems & Nanoengineering》 (微系统与纳米工程（英文）)
年 卷 期：2019年第5卷第1期
页 面：50-58页
核心收录：
学科分类：0808[工学-电气工程] 1002[医学-临床医学] 0809[工学-电子科学与技术（可授工学、理学学位）] 0817[工学-化学工程与技术] 0807[工学-动力工程及工程热物理] 0805[工学-材料科学与工程（可授工学、理学学位）] 0802[工学-机械工程] 100214[医学-肿瘤学] 0811[工学-控制科学与工程] 0702[理学-物理学] 10[医学]
基 金：Work supported by NSF(CBET-1604562),NIH(1R21 ES029791) World anti-doping agent(WADA)and Partnership for Clean Competition(PCC)
主 题：impaired fluid transfusion
摘 要：Changes in the deformability of red blood cells can reveal a range of *** example,cells which have been stored for transfusion are known to exhibit progressively impaired ***,this aspect of red blood cells has been characterized previously using a range of *** this paper,we show a novel approach for examining the biophysical response of the cells with quantitative phase ***,optical volume changes are observed as the cells transit restrictive channels of a microfluidic chip in a high refractive index *** optical volume changes indicate an increase of cell’s internal density,ostensibly due to water ***,we characterize these changes over time for red blood cells from two *** storage day 29,a significant decrease in the magnitude of optical volume change in response to mechanical stress was *** exchange of water with the environment due to mechanical stress is seen to modulate with storage time,suggesting a potential means for studying cell storage.
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