Maternal betaine suppresses adrenal expression of cholesterol trafficking genes and decreases plasma corticosterone concentration in offspring pullets

作     者：Halima Abobaker Yun Hu Nagmeldin A.Omer Zhen Hou Abdulrahman A.Idriss Ruqian Zhao Halima Abobaker;Yun Hu;Nagmeldin A. Omer;Zhen Hou;Abdulrahman A. Idriss;Ruqian Zhao

作者机构：MOE Joint International Research Laboratory of Animal Health&Food SafetyNanjing Agricultural UniversityNanjing 210095People’s Republic of China Key Laboratory of Animal Physiology&BiochemistryNanjing Agricultural UniversityNanjing 210095People’s Republic of China College of Allied Medical SciencesUniversity of Nyala155 NyalaSudan. 

出 版 物：《Journal of Animal Science and Biotechnology》 (畜牧与生物技术杂志（英文版）)

年 卷 期：2020年第11卷第1期

页      面：241-250页

核心收录：

学科分类：090502[农学-动物营养与饲料科学] 0905[农学-畜牧学] 09[农学] 

基　　金：supported by the National Natural Science Foundation of China(31672512) the Fundamental Research Funds for the Central Universities(KYZ201212) the Priority Academic Program Development of Jiangsu Higher Education Institutions(PAPD) Jiangsu Collaborative Innovation Centre of Meat Production and Processing,Quality and Safety Control 

主　　题：Adrenal gland Chicken Cholesterol Corticosterone Maternal betaine Steroidogenesis 

摘      要：Background:Laying hens supplemented with betaine demonstrate activated adrenal steroidogenesis and deposit higher corticosterone(CORT)in the egg *** we further investigate the effect of maternal betaine on the plasma CORT concentration and adrenal expression of steroidogenic genes in offspring ***:Maternal betaine significantly reduced(P0.05)plasma CORT concentration and the adrenal expression of vimentin that is involved in trafficking cholesterol to the mitochondria for utilization in offspring ***,voltage-dependent anion channel 1 and steroidogenic acute regulatory protein,the two mitochondrial proteins involved in cholesterol influx,were both down-regulated at m RNA and protein ***,enzymes responsible for steroid syntheses,such as cytochrome P450 family 11 subfamily A member 1 and cytochrome P450 family 21 subfamily A member 2,were significantly(P0.05)up-regulated at m RNA or protein levels in the adrenal gland of pullets derived from betaine-supplemented ***,expression of transcription factors,such as steroidogenic factor-1,sterol regulatory element-binding protein 1 and c AMP response element-binding protein,was significantly(P0.05)enhanced,together with their downstream target genes,such as 3-hydroxy-3-methyl-glutaryl-coenzyme A reductase,LDL receptor and sterol regulatory element-binding protein cleavage-activating *** promoter regions of most steroidogenic genes were significantly(P0.05)hypomethylated,although methyl transfer enzymes,such as AHCYL,GNMT1 and BHMT were ***:These results indicate that the reduced plasma CORT in betaine-supplemented offspring pullets is linked to suppressed cholesterol trafficking into the mitochondria,despite the activation of cholesterol and corticosteroid synthetic genes associated with promoter hypomethylation.