Association of glypican-3 expression with growth signaling molecules in hepatocellular carcinoma
Association of glypican-3 expression with growth signaling molecules in hepatocellular carcinoma作者机构：First Department of Internal MedicineSapporo Medical University School of MedicineSapporo 060-8543Japan Sapporo Medical UniversitySapporo 060-8556 Japan
出 版 物：《World Journal of Gastroenterology》 (世界胃肠病学杂志（英文版）)
年 卷 期：2010年第16卷第28期
页 面：3521-3528页
核心收录：
学科分类：1002[医学-临床医学] 100214[医学-肿瘤学] 10[医学]
基 金：Supported by Grants-in-Aid for Scientific Research from the Ministry of Education,Culture,Sports,Science and Technology of Japan(to Yamamoto H,Imai K and Shinomura Y) Grants-in-Aid for Cancer Research from the Ministry of Health,Labor and Welfare of Japan(to Yamamoto H)
主 题：Glypican-3 Wnt Matrix metalloproteinases Sulfatase Hepatocellular carcinoma
摘 要：AIM:To clarify the association of glypican-3(GPC3)expression with Wnt and other growth signaling molecules in hepatocellular carcinoma(HCC). METHODS:Expression of GPC3,Wnt,matrix metalloproteinases(MMPs),sulfatase(SULF)1,SULF2,and other growth signaling molecules was analyzed in HCC cell lines and tissue samples by real-time reverse transcriptionpolymerase chain reaction,immunoblotting,and/or *** of various genes in GPC3 siRNA-transfected HCC cells was analyzed. RESULTS:GPC3 was overexpressed in most HCCs at mRNA and protein levels and its serum levels weresignificantly higher in patients with HCC than in non- HCC subjects(P0.05).Altered expressions of various MMPs and growth signaling molecules,some of which were correlated with GPC3 expression,were observed in ***-regulation of GPC3 expression by siRNA in GPC3-overexpressing HCC cell lines resulted in a significant decrease in expressions of MMP2,MMP14,fibroblast growth factor receptor 1,insulin-like growth factor 1 ***3 expression was significantly correlated with nuclear/cytoplasmic localization ofβ-catenin. CONCLUSION:These results suggest that GPC3,in conjunction with MMPs and growth signaling molecules, might play an important role in the progression of HCC.
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