Ubiquitin-conjugating enzyme E2T knockdown suppresses hepatocellular tumorigenesis via inducing cell cycle arrest and apoptosis

作     者：Jian Guo Mu Wang Jun-Ping Wang Chang-Xin Wu 

作者机构：Institute of BiotechnologyKey Laboratory of Chemical Biology and Molecular Engineering of Ministry of EducationInstitute of BiotechnologyShanxi UniversityTaiyuan 030006Shanxi ProvinceChina Department of General SurgeryShanxi Provincial People’s Hospitalthe Affiliated People’s Hospital of Shanxi Medical UniversityTaiyuan 030012Shanxi ProvinceChina Department of NeurologyShanxi Provincial People’s Hospitalthe Affiliated People’s Hospital of Shanxi Medical UniversityTaiyuan 030012Shanxi ProvinceChina Department of GastroenterologyShanxi Provincial People’s Hospitalthe Affiliated People’s Hospital of Shanxi Medical UniversityTaiyuan 030001Shanxi ProvinceChina The Institutes of Biomedical SciencesShanxi UniversityTaiyuan 03006Shanxi ProvinceChina Key Laboratory of Molecular Biology and Biochemistry of Ministry of EducationShanxi UniversityTaiyuan 030006Shanxi ProvinceChina 

出 版 物：《World Journal of Gastroenterology》 (世界胃肠病学杂志（英文版）)

年 卷 期：2019年第25卷第43期

页      面：6386-6403页

核心收录：

学科分类：1002[医学-临床医学] 100214[医学-肿瘤学] 10[医学] 

主　　题：Hepatocellular carcinoma Ubiquitin-conjugating enzyme E2T Cell cycle Apoptosis Tumorigenesis 

摘      要：BACKGROUND Hepatocellular carcinoma(HCC)is now the most common primary liver malignancy worldwide,and multiple risk factors attribute to the occurrence and development of ***,increasing studies suggest that ubiquitinconjugating enzyme E2T(UBE2T)serves as a promising prognostic factor in human cancers,although the molecular mechanism of UBE2T in HCC remains *** To investigate the clinical relevance and role of UBE2T in HCC *** UBE2T expression in HCC tissues from the TCGA database and its association with patient survival were analyzed.A lentivirus-mediated strategy was used to knock down UBE2T in HCC ***-PCR and Western blot assays were performed to check the effect of UBE2T silencing in HCC *** growth in vitro and in vivo was analyzed by multiparametric high-content screening and the xenograft tumorigenicity assay,*** cycle distribution and apoptosis were determined by flow *** genes regulated by UBE2T were profiled by microarray *** UBE2T was overexpressed in HCC tissues compared with paired and non-paired normal *** expression of UBE2T predicted a poor overall survival in HCC *** vitro,lentivirus-mediated UBE2T knockdown significantly reduced the viability of both SMMC-7721 and BEL-7404 *** vivo,the xenograft tumorigenesis of SMMC-7721 cells was largely attenuated by UBE2T *** cell cycle was arrested at G1/S phase in SMMC-7721 and BEL-7404 cells with UBE2T ***,apoptosis was increased by UBE2T *** the molecular level,numerous genes were dysregulated after UBE2T silencing,including IL-1B,FOSL1,PTGS2,and *** UBE2T plays an important role in cell cycle progression,apoptosis,and HCC development.