MATRIEX imaging:multiarea two-photon real-time in vivo explorer

作     者：Mengke Yang Zhenqiao Zhou Jianxiong Zhang Shanshan Jia Tong Li Jiangheng Guan Xiang Liao Bing Leng Jing Lyu Kuan Zhang Min Li Yan Gong Zhiming Zhu Junan Yan Yi Zhou Jian K Liu Zsuzsanna Varga Arthur Konnerth Yuguo Tang Jinsong Gao Xiaowei Chen Hongbo Jia 

作者机构：Key Laboratory of Optical System Advanced Manufacturing TechnologyChangchun Institute of OpticsFine Mechanics and PhysicsChinese Academy of SciencesChangchun 130033China Graduate SchoolUniversity of the Chinese Academy of SciencesBeijing 100039China Brain Research Instrument Innovation CenterSuzhou Institute of Biomedical Engineering and TechnologyChinese Academy of SciencesSuzhou 215163China Brain Research Center and State Key Laboratory of TraumaBurnsand Combined InjuryThird Military Medical UniversityChongqing 400038China Center for NeurointelligenceChongqing UniversityChongqing 401331China Center for Hypertension and Metabolic DiseasesDaping HospitalChongqing 400042China Advanced Institute of Brain and IntelligenceGuangxi UniversityNanning 530005China Centre for Systems NeuroscienceDepartment of NeurosciencePsychology and BehaviourUniversity of LeicesterLeicesterUK Institute of NeuroscienceTechnical University Munich80802 MunichGermany 

出 版 物：《Light(Science & Applications)》 (光（科学与应用)（英文版）)

年 卷 期：2019年第8卷第1期

页      面：156-166页

核心收录：

学科分类：070207[理学-光学] 07[理学] 08[工学] 0803[工学-光学工程] 0702[理学-物理学] 

基　　金：supported by the“100-Talents Program for Elite Engineers”of the CAS(H.J.) the Key Scientific Research Equipment Development Project of the CAS(Super-resolution Microscopy Systems and Key Components,ZDYZ2013-1) the“1000-Talents Program for Young Scholars”of China(X.C.) grants from the Ministry of Science and Technology of China(“973 Program”:2015CB759500,2018YFA0109600) the National Natural Science Foundation of China(61705251,81671106,81771175,31700933,81721001) the China Postdoctoral Science Foundation(2018M632374) 

主　　题：photon readily typically 

摘      要：Two-photon laser scanning microscopy has been extensively applied to study in vivo neuronal activity at cellular and subcellular resolutions in mammalian ***,the extent of such studies is typically confined to a single functional region of the ***,we demonstrate a novel technique,termed the multiarea two-photon real-time in vivo explorer(MATRIEX),that allows the user to target multiple functional brain regions distributed within a zone of up to 12mm in diameter,each with a field of view(FOV)of ~200μm in diameter,thus performing two-photon Ca2+imaging with single-cell resolution in all of the regions *** example,we demonstrate real-time functional imaging of single-neuron activities in the primary visual cortex,primary motor cortex and hippocampal CA1 region of mice in both anesthetized and awake states.A unique advantage of the MATRIEX technique is the configuration of multiple microscopic FOVs that are distributed in three-dimensional space over macroscopic distances(1 mm)both laterally and axially but that are imaged by a single conventional laser scanning *** particular,the MATRIEX technique can be effectively implemented as an add-on optical module for an existing conventional single-beam-scanning two-photon microscope without requiring any modification to the microscope ***,the MATRIEX technique can be readily applied to substantially facilitate the exploration of multiarea neuronal activity in vivo for studies of brain-wide neural circuit function with single-cell resolution.