MicroRNA-30c inhibits pancreatic cancer cell proliferation by targeting twinfilin 1 and indicates a poor prognosis

作     者：Lu-Lu Sun Ming Cheng Xiao-Dong Xu 

作者机构：Department of Ultrasonographythe First Affiliated Hospital of Zhengzhou UniversityZhengzhou 450000Henan ProvinceChina Department of Informationthe First Affiliated Hospital of Zhengzhou UniversityZhengzhou 450000Henan ProvinceChina Department of Breast Surgerythe First Affiliated Hospital of Zhengzhou UniversityZhengzhou 450000Henan ProvinceChina 

出 版 物：《World Journal of Gastroenterology》 (世界胃肠病学杂志（英文版）)

年 卷 期：2019年第25卷第42期

页      面：6311-6321页

核心收录：

学科分类：1002[医学-临床医学] 100214[医学-肿瘤学] 10[医学] 

基　　金：Supported by the National Nature Science Foundation of China No.61802350 

主　　题：Pancreatic cancer MicroRNA-30c Proliferation Twinfilin 1 

摘      要：BACKGROUND Studies have reported that microRNA-30c(miR-30c)has vital functions in the development and progression of multiple *** To investigate the clinical significance and role of miR-30c in pancreatic *** MiR-30c and twinfilin 1(TWF1)expression levels were analyzed in Gene Expression Omnibus datasets and validated in human pancreatic cancer by quantitative real-time polymerase chain reaction(RT-qPCR).The effects of miR-30c on pancreatic cancer cell growth,apoptosis,and cell cycle were evaluated by CCK-8 and flow cytometry ***,the in vivo effects were investigated using a subcutaneous xenograft *** gene prediction software and luciferase reporter assays were used to identify TWF1 as a direct target of *** The expression of miR-30c was significantly decreased in pancreatic cancer tissues and associated with ***-and loss-of-function assays showed that miR-30c suppressed pancreatic cancer cell proliferation in vitro and in ***-qPCR,Western blot,and luciferase reporter assays showed that miR-30c directly targeted *** expression level of miR-30c was negatively correlated with TWF1 expression in pancreatic cancer ***,the effects of ectopic miR-30c were rescued by TWF1 *** Our results identified the role of the miR-30c/TWF1 axis in pancreatic cancer progression and demonstrated that miR-30c might serve as a prognostic biomarker and therapeutic target for pancreatic cancer.