Expression of Erk kinase,AMPA receptor subunits GluR1 and GluR2,and protection of chondroitin sulfate in brain of rats with chronic fluorosis

作     者：WANG Chun 王纯

作者机构：Dept Pathol Affili Hosp Guizhou Med Univ 

出 版 物：《China Medical Abstracts(Internal Medicine)》 (中国医学文摘（内科学分册（英文版）)

年 卷 期：2019年第36卷第3期

页      面：141-142页

学科分类：1002[医学-临床医学] 100214[医学-肿瘤学] 10[医学] 

主　　题：GluR1 GluR2 rats 

摘      要：Objective To study the mechanism of central nervous system(CNS)injury in chronic fluorosis and the neuroprotective effect of chondroitin sulfate(CS).Methods Forty-eight female Sprague-Dawley rats weighting 90-120 g were divided into 8 groups according to body weight by random number table,6 rats in each group:control group,drinking tap water freely;low dose and high dose fluoride groups,freely drinking tap water with fluoride content of 10 and 50 mg/L,respectively;control+normal saline(NS),low dose fluoride+NS,and high dose fluoride+NS groups,each group was fed for 180 d,and treated with intraperitoneal injection of 0.66 mg/kg NS for 5 d(once a day);low dose fluoride+CS and high dose fluoride+CS groups,each group was fed for 180 d,0.66 mg/kg CS was injected intraperitoneally for 5 d(once a day).All groups were fed standard nutritive animal feed for 185 d and dissected for brain *** pathologic change was observed after hematoxylin-eosin(HE)staining;the expression levels of phosphorylated extracellular signal-regulated protein kinase 1/2(phospho-Erk1/2)and glutamate receptors 1,2(GluR1,GluR2)in the brain cortex were detected by immunohistochemistry;the protein levels of Erk1/2,phospho-Erk1/2,GluR1,and GluR2 in the brain cortex were detected by Western *** Brain cortex of all rats in the fluoride groups showed eosinophilic degeneration,loss and disordered arrangement of neurons,and the brain morphological changes in each fluoride+CS groups were significantly improved compared with those in the fluoride *** results showed that compared with the control group[(0.44±0.09)%,(1.49±0.05)%,(2.51±0.54)%],the expression levels of phospho-Erk1/2[(1.47±0.09)%,(1.03±0.05)%],and GluR2[(2.37±0.06)%,(3.38±0.12)%]in the low dose and high dose fluoride groups were increased,and the expression levels of GluR1[(1.49±0.02)%,(0.99±0.19)%]were decreased(P0.05).Western blotting results showed that compared with the control group(1.00±0.12,1.76±0.3