Study of Bcl-2 siRNA Enhancement of Sensitivity of HL-60 Cells to All Trans Retinoic Acid

作     者：Haiyan Hu Yuan Zhang Dongmei He 

作者机构：Institute of Hematology Medical College Jinan University Guangzhou 510632 Guangdong province China. 

出 版 物：《Chinese Journal of Clinical Oncology》 (中国肿瘤临床（英文版）)

年 卷 期：2008年第5卷第2期

页      面：118-121页

核心收录：

学科分类：1007[医学-药学(可授医学、理学学位)] 1006[医学-中西医结合] 100706[医学-药理学] 100602[医学-中西医结合临床] 10[医学] 

基　　金：The work was supported by a grant from the Key Subject Foundation of Overseas Chinese Affairs Office of the State Council(No.51205002) 

主　　题：Bcl-2 siRNA all trans retinoic acid (ATRA) drug-sensitivity. 

摘      要：OBJECTIVE To study whether siRNA targeting against the Bcl-2 gene can enhance sensitivity of HL-60 cells to all trans retinoic acid(ATRA). METHODS siRNA,which is a leading sequence selected by previous experiments,was transferred into HL-60 *** 6 h after transfection,the cells were cultured with *** cell growth of the HL-60 cells was measured by the MTT assay at 24, 48,72 *** level of the Bcl-2 protein and ROS(reactive oxygen species)as well as membrane potential of the mitochondria were determined by flowcytometry. RESULTS siRNA significantly increased the inhibitory effect of ATRA on growth of the HL-60 *** combination of siRNA with ATRA resulted in a decrease in the Bcl-2 protein level and an increase in the ROS level as well as significantly lowering the mitochondrial membrane potential of the HL-60 cells(P0.05). CONCLUSION Effective siRNA targeting of Bcl-2 increases the sensitivity of HL-60 leukemic cells to ATRA by inhibiting the expression of the Bcl-2 protein.