Prokineticin 2 overexpression induces spermatocyte apoptosis in varicocele in rats
Prokineticin 2 overexpression 在老鼠在精索静脉曲张导致 spermatocyte apoptosis作者机构：Family Planning Research Institute/Reproductive Medicine CenterTongji Medical CollegeHuazhong University of Science and TechnologyWuhan 430030China Departm ent of Gynecology and ObstetricsUnion HospitalTorigji Medical CollegeHuangzhong University of Science and TechnologyWuhan 430030China
出 版 物：《Asian Journal of Andrology》 (亚洲男性学杂志（英文版）)
年 卷 期：2020年第22卷第5期
页 面：500-506页
核心收录：
学科分类：1002[医学-临床医学] 100210[医学-外科学(含：普外、骨外、泌尿外、胸心外、神外、整形、烧伤、野战外)] 10[医学]
基 金：the National Natural Science Foundation of China(Grant No.81871148 No.81701539 and No.81571496)
主 题：apoptosis oxidative stress prokineticin 2 varicocele
摘 要：Varicocele is one of the most important causes of male infertility,as this condition leads to a decline in sperm *** is generally believed that the presence of varicocele induces an increase in reactive oxygen species levels,leading to oxidative stress and sperm apoptosis;however,the specific pathogenic mechanisms affecting spermatogenesis remain *** 2(PK2),a secretory protein,is associated with multiple biological processes,including cell migration,proliferation,and *** the testis,PK2 is expressed in spermatocytes under normal physiological *** investigate the role of PK2 in varicocele,a rat varicocele model was established to locate and quantify the expression of PK2 and its receptor,prokineticin receptor 1(PKR1),by immunohistochemistry and quantitative real-time PCR assays(qPCR).Moreover,H2O2 was applied to mimic the oxidative stress state of varicocele through coculturing with a spermatocyte-derived cell line(GC-2)in vitro,and the apoptosis rate was detected by flow ***,we illustrated that the expression levels of PK2 and PKR1 were upregulated in the spermatocytes of the rat *** of H202 stimulated the overexpression of PK2 in *** of recombinant pCMV-HA-PK2 into GC-2 cells promoted apoptosis by upregulating cleaved-caspase-3,caspase-8,and B cell lymphoma 2-associated X;downregulating B cell lymphoma 2;and promoting the accumulation of intracellular ***,we revealed that the varicocele-induced oxidative stress stimulated the overexpression of PK2,leading to apoptosis of *** study provides new insight into the mechanisms underlying oxidative stress-associated male infertility and suggests a novel therapeutic target for male infertility.
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