The expression of MAPK signaling pathways in conjunctivochalasis

作     者：Yuan-Ling Jia Xiao-Jing Liu Hang Wen Yue-Ping Zhan Min-Hong Xiang 

作者机构：Department of Ophthalmology Putuo Hospital Shanghai University of Traditional Chinese Medicine 

出 版 物：《International Journal of Ophthalmology(English edition)》 (国际眼科杂志（英文版）)

年 卷 期：2019年第12卷第11期

页      面：1801-1806页

核心收录：

学科分类：1002[医学-临床医学] 100214[医学-肿瘤学] 10[医学] 

基　　金：Supported by the Research Project of Health and Family Planning Commission in Shanghai(No.201840196) Yingcai Program of Putuo Hospital(No.2017202B) Yuying Program of Putuo Hospital(No.2016219A) 

主　　题：ERK JNK p38 MAPK conjunctivochalasis phosphorylation level 

摘      要：This study investigated the potential role of MAPK signaling pathways in conjunctivochalasis(CCH). Twenty loose conjunctival biopsy samples from 20 CCH and 15 conjunctival biopsy samples from 15 normal controls(CON) were collected. The conjunctival fibroblasts were cultured in vitro. Immunofluorescence, ELISA, Western blot and reverse transcription-polymerase chain reaction(RT-PCR) were used. Our results showed that the expression of p-ERK, p-JNK, and p-p38 in CCH conjunctiva was significantly higher than that in CON group. The expression of p38 MAPK, JNK, and ERK proteins in CCH fibroblasts was significantly higher than that in CON group. The total expression of MAPK m RNA in CCH fibroblasts was significantly higher than that in CON group. The activated forms of p38 MAPK, JNK, and ERK proteins and m RNAs might up-regulate the expression of MMPs in CCH loose conjunctival tissue and fibroblasts, causing the degradation of collagen fibers and elastic fibers and promoting the occurrence of CCH. Our results deepen the understanding of CCH pathological mechanism.