Contrasting Effects of Ethylene Biosynthesis on Induced Plant Resistance against a Chewing and a Piercing-Sucking Herbivore in Rice

作     者：Jing Lu Jiancai Li Hongping Ju Xiaoli Liu Matthias Erb Xia Wang Yonggen Lou 

作者机构：State Key Laboratory of Rice Biology Institute of Insect Sciences Zhejiang University Hangzhou 310058 China Institute of Plant Sciences University of Bern Altenbergrain 21 3013Bern Switzerland 

出 版 物：《Molecular Plant》 (分子植物（英文版）)

年 卷 期：2014年第7卷第11期

页      面：1670-1682页

核心收录：

学科分类：0710[理学-生物学] 09[农学] 0904[农学-植物保护] 0901[农学-作物学] 090401[农学-植物病理学] 0902[农学-园艺学] 090402[农学-农业昆虫与害虫防治] 090201[农学-果树学] 

基　　金：The study was jointly sponsored by the National Basic Research Program of China (2010CB126200)  the Innovation Research Team Program of the National Natural Science Foundation of China (31321063)  the National Program of Transgenic Variety Development of China (2011ZX08001- 001)  and the China Agriculture Research System (CARS-01-21) 

主　　题：rice OsACS2 ethylene direct defense indirect defense herbivore resistance. 

摘      要：Ethylene is a stress hormone with contrasting whether these differences are plant- or herbivore-specific. effects on herbivore resistance. However, it remains unknown We cloned a rice 1-aminocyclopropane-l-carboxylic acid （ACC） synthase gene, OsACS2, whose transcripts were rapidly up-regulated in response to mechanical wounding and infestation by two important pests： the striped stem borer （SSB） Chilo suppressalis and the brown planthopper （BPH） Nilaparvata lugens. Antisense expression of OsACS2 （as-acs） reduced elicited ethylene emission, SSB-elicited trypsin protease inhibitor （TrypPI） activity, SSB-induced volatile release, and SSB resistance. Exogenous application of ACC restored TrypPI activity and SSB resistance. In contrast to SSB, BPH infestation increased volatile emission in as-acs lines. Accordingly, BPH preferred to feed and oviposit on wild-type （WT） plants--an effect that could be attributed to two repellent volatiles, 2-hep- tanone and 2-heptanol, that were emitted in higher amounts by as-acs plants. BPH honeydew excretion was reduced and natural enemy attraction was enhanced in as-acs lines, resulting in higher overall resistance to BPH. These results demonstrate that ethylene signaling has contrasting, herbivore-specific effects on rice defense responses and resistance against a chewing and a piercing-sucking insect, and may mediate resistance trade-offs between herbivores of different feeding guilds in rice.