A WRKY transcription factor confers aluminum tolerance via regulation of cell wall modifying genes

作     者：Chun Xiao Li Jing Ying Yan Jiang Yuan Ren Li Sun Chen Xu Gui Xin Li Zhong Jie Ding Shao Jian Zheng Chun Xiao Li;Jing Ying Yan;Jiang Yuan Ren;Li Sun;Chen Xu;Gui Xin Li;Zhong Jie Ding;Shao Jian Zheng

作者机构：State Key Laboratory of Plant Physiology and BiochemistryCollege of Life SciencesZhejiang UniversityHangzhou 310058China Agricultural Experiment StationZhejiang UniversityHangzhou 310058China College of Agronomy and BiotechnologyZhejiang UniversityHangzhou 310058China 

出 版 物：《Journal of Integrative Plant Biology》 (植物学报（英文版）)

年 卷 期：2020年第62卷第8期

页      面：1176-1192页

核心收录：

学科分类：0710[理学-生物学] 07[理学] 09[农学] 071007[理学-遗传学] 0901[农学-作物学] 0703[理学-化学] 0902[农学-园艺学] 090102[农学-作物遗传育种] 

基　　金：This work was supported by the Ministry of Science and Technology of China(Grant No.2015CB942903) the National Natural Science Foundation of China(Grant No.31500206,31730006) China Postdoctoral Science Foun-dation(Grant No.2017T100428) 111 project(Grant No.B14027) the Fundamental Research Funds for the Central Universities 

主　　题：distribution WRKY tolerance 

摘      要：Modification of cell wall properties has been considered as one of the determinants that confer aluminum(Al)tolerance in plants,while how cell wall modifying processes are regulated remains ***,we present a WRKY transcription factor WRKY47 involved in Al tolerance and root *** of WRKY47 significantly reduces,while overexpression of it increases Al *** show that lack of WRKY47 substantially affects subcellular Al distribution in the root,with Al content decreased in apoplast and increased in symplast,which is attributed to the reduced cell wall Al-binding capacity conferred by the decreased content of hemicellulose I in the wrky47-1 *** on microarray,real time-quantitative polymerase chain reaction and chromatin immunoprecipitation assays,we further show that WRKY47 directly regulates the expression of EXTENSIN-LIKE PROTEIN(ELP)and XYLOGLUCAN ENDOTRANSGLUCOSYLASE-HYDROLASES17(XTH17)responsible for cell wall *** the expression of ELP and XTH17 rescued Al tolerance as well as root growth in wrky47-1 *** summary,our results demonstrate that WRKY47 is required for root growth under both normal and Al stress conditions via direct regulation of cell wall modification genes,and that the balance of Al distribution between root apoplast and symplast conferred by WRKY47 is important for Al tolerance.