Mannotriose regulates learning and memory signal transduction in the hippocampus

作     者：Lina Zhang Weiwei Dai Xueli Zhang Zhangbin Gong Guoqin Jin 

作者机构：Department of BiochemistryInstitute of Basic MedicineShanghai University of Traditional Chinese Medicine 

出 版 物：《Neural Regeneration Research》 (中国神经再生研究（英文版）)

年 卷 期：2013年第8卷第32期

页      面：3020-3026页

核心收录：

学科分类：0710[理学-生物学] 1002[医学-临床医学] 1001[医学-基础医学(可授医学、理学学位)] 07[理学] 071006[理学-神经生物学] 

基　　金：financially supported by the National Natural Science Foundation of China,No.30873317 the Leading Academic Discipline Project of Shanghai Municipal Education Commission,No.J50301 "085 Class Disciplines Building"Scientific and Technology Innovation Fundation,No.085ZY1204 Shanghai Health Bureau of Scientific Research Projects,No.20124Y025 

主　　题：neural regeneration traditional Chinese medicine Rehmannia mannotriose corticosterone hippocampus neurons learning and memory grants-supported paper neuroregeneration 

摘      要：Rehmannia is a commonly used Chinese herb, which improves ieaming and memory. However, the crucial components of the signal transduction pathway associated with this effect remain elusive. Pri- mary hippocampal neurons were cultured in vitro, insulted with high-concentration （1 × 10-4 mol/L） cor- ticosterone, and treated with 1 × 104 mol/L mannotriose. Thiazolyl blue tetrazolium bromide assay and western blot analysis showed that hippocampal neuron survival rates and protein levels of glucocorti- cold receptor, serum and glucocorticoid-regulated protein kinase, and brain-derived neurotrophic factor were all dramatically decreased after high-concentration corticosterone-induced injury. This effect was reversed by mannotriose, to a similar level as RU38486 and donepezil. Our findings indicate that mannotriose could protect hippocampal neurons from high-concentration corticosterone-induced injury. The mechanism by which this occurred was associated with levels of glucocorticoid receptor protein, serum and glucocorticoid-regulated protein kinase, and brain-derived neurotrophic factor.