LjCYC Genes Constitute Floral Dorsoventral Asymmetry in Lotus japonicus

作     者：Jiechen Wang Yumei Wang Da Luo 

作者机构：National Key Laboratory of Plant Molecular GeneticsInstitute of Plant Physiology and EcologyShanghai Institutes for Biological Sciencesthe Chinese Academy of SciencesShanghai 200032China Graduate University of Chinese Academy of SciencesBeijing 100049China Department of Cell BiologyShanghai Jiaotong University Medical of SchoolShanghai 200025China 

出 版 物：《Journal of Integrative Plant Biology》 (植物学报（英文版）)

年 卷 期：2010年第52卷第11期

页      面：959-970页

核心收录：

学科分类：090503[农学-草业科学] 0909[农学-草学] 082902[工学-木材科学与技术] 0905[农学-畜牧学] 08[工学] 09[农学] 0829[工学-林业工程] 

基　　金：supported by a grant (2009ZX08009-112B) from the Ministry of Agriculture,China the National Natural Science Foundation of China (30930009) 

主　　题：GENES LEGUMES HEREDITY RNA TRANSGENES 

摘      要：Previous study shows that LjCYC2,a CYC-like TCP （TB1,CYC and PCFs） gene in the model legume,Lotus japonicus,is involved in dorsal petal development,which together with the other two homologous genes,LjCYC1 and LjCYC3,belongs to an LjCYC gene *** this report,we modified the transformation system in ***,and constructed different RNAi transgenes to target different LjCYC *** expression of three endogenous LjCYC genes was specifically suppressed by different specific RNAi transgenes,and a chimerical RNAi transgene that contains the specific sequences from LjCYC1 and LjCYC2 was found to downregulate the expression of both endogenous genes *** of silencing three LjCYC genes were mainly restricted on either dorsal or lateral petals,demonstrating their dorsalizing and lateralizing activities during the development of zygomorphic ***,abolishing the expression of three LjCYC genes could give rise to complete loss of dorsoventral （DV） differentiation in the flower whose petals all resembled the ventral one in the wild type and displayed intact organ internal （IN） *** data demonstrate that during zygomorphic flower development,the DV asymmetry is constituted by the LjCYC genes,while the floral organ IN asymmetry is independently determined by other genetic factors.