In vitro effects of chitosan nanoparticles on proliferation of human gastric carcinoma cell line MGC803 cells
In vitro effects of chitosan nanoparticles on proliferation of human gastric carcinoma cell line MGC803 cells作者机构：Nanobiology Laboratory of Animal Science College Zhejiang UniversityHangzhou 310029 Zhejiang Province China
出 版 物：《World Journal of Gastroenterology》 (世界胃肠病学杂志（英文版）)
年 卷 期：2005年第11卷第33期
页 面：5136-5141页
核心收录：
学科分类：10[医学]
主 题：Chitosan nanoparticles Gastric carcinoma Necrosis Apoptosis
摘 要：AIM： To investigate the effects of chitosan nanoparticles on proliferation of human gastric carcinoma cell line MGC803 in vitro and the possible mechanisms involved. METHODS： Chitosan nanoparticles were characterized by particle size, zeta potential, and morphology. After treatment with various concentrations of chitosan nanoparticles （25, 50, 75, 100 μg/mL） at various time intervals, cell proliferation, ultrastructural changes, DNA fragmentation, mitochondrial membrane potential （MMP）, cell cycle phase distribution and apoptotic peaks of MGC803 cells were analyzed by MTT assay, electron microscopy, DNA agarose gel electrophoresis, and flow cytometry. RESULTS： Chitosan nanoparticles exhibited a small particle size as 65 nm and a high surface charge as 52 mV. Chitosan nanoparticles markedly inhibited cell proliferation of MGC803 cells with an ICso value of 5.3 μg/mL 48 h after treatment. After treatment with chitosan nanoparticles, the typical necrotic cell morphology was observed by electron microscopy, a typical DNA degradation associated with necrosis was determined by DNA agarose electrophoresis. Flow cytometry showed the loss of MMP and occurrence of apoptosis in chitosan nanoparticles-treated cells. CONCLUSION： Chitosan nanoparticles effectively inhibit the proliferation of human gastric carcinoma cell line MGC803 in vitro through multiple mechanisms, and may be a beneficial agent against human carcinoma.
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